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פותח על ידי קלירמאש פתרונות בע"מ -
Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR
Year:
2017
Source of publication :
Phytoparasitica
Authors :
בורנשטיין, מנחם
;
.
ברומין, מרינה
;
.
גאבה, ויקטור
;
.
גל-און, עמית
;
.
ליבמן, דיאנה
;
.
סלע, נעה
;
.
פרלסמן, מלניה
;
.
תם, יהודית
;
.
Volume :
Co-Authors:
Zeidan, Mouhammad
Dar, Zion
Zig, Uri
Facilitators :
From page:
1
To page:
8
(
Total pages:
8
)
Abstract:
Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the “Growing-On test”), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013–2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate (<5% by Growing On test; suitable for seed) and those unsuitable for seed (≥5% by Growing On test). Therefore, qPCR is an appropriate technique for determination of the PVY infection rate of seed tuber lots in Israel.
Note:
Related Files :
Growing-On test
Potato virus Y
PVY
Real Time PCR
Seed potatoes
virus detection
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
Editors' remarks:
ID:
18369
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:18
Scientific Publication
Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR
Zeidan, Mouhammad
Dar, Zion
Zig, Uri
Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR
Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the “Growing-On test”), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013–2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate (<5% by Growing On test; suitable for seed) and those unsuitable for seed (≥5% by Growing On test). Therefore, qPCR is an appropriate technique for determination of the PVY infection rate of seed tuber lots in Israel.
Scientific Publication
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