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פותח על ידי קלירמאש פתרונות בע"מ -
Prevalence of internalisation-associated gene, prtF1, among persisting group-A streptococcus strains isolated from asymptomatic carriers
Year:
1998
Source of publication :
Lancet
Authors :
סלע, שלמה
;
.
Volume :
352
Co-Authors:
Neeman, R.
Keller, N., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Barzilai, A., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Korenman, Z., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 69978, Israel
Facilitators :
From page:
1974
To page:
1977
(
Total pages:
4
)
Abstract:
Background. The failure of antibiotic treatment to eradicate group-A streptococci in up to 30% of patients with pharyngotonsillitis is unexplained. Some strains of group-A streptococci can enter respiratory epithelial cells, where they would be inaccessible to antibiotics unable to penetrate the cell membrane, such as penicillins. The fibronectin-binding proteins, F1 and SfbI, are needed for this process. We hypothesised, therefore, that an intracellular reservoir of group-A streptococci could account, at least partly, for failure to eradicate throat carriage, and that the presence of the gene for fibronectin-binding protein (F1) might be linked to the ability of a strain to persist in the throat after therapy. Methods. We investigated the frequency of prtF1-containing strains among 67 patients with pharyngotonsillitis. All patients were clinically cured, although 13 of them continued to carry group-A streptococci in the throat during or after therapy. To distinguish between persisting and recolonising strains, isolates from the 13 patients were serologically tested and compared by polymorphic DNA-amplification technique. Findings. 12 (92%) of the 13 patients with symptomless had prtF1-containing strains in the throat, with 16 (30%) of the 54 patients with eradication (p = 0.0001). Three of the 13 eradication-failure patients were recolonised with strains that differed from the pretreatment strains. Nine of the ten (90%) persisting strains carried prtF1 (p = 0.0009). Interpretation. Our findings suggest that protein-F1-mediated entry to cells is involved in the causative process of the carriage state.
Note:
Related Files :
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עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0140-6736(97)12452-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18392
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
Scientific Publication
Prevalence of internalisation-associated gene, prtF1, among persisting group-A streptococcus strains isolated from asymptomatic carriers
352
Neeman, R.
Keller, N., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Barzilai, A., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Korenman, Z., Departments of Clin. M. and P., Chalm Sheba Medical Center, Tel-Hashomer Hospital, Jerusalem, Israel
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 69978, Israel
Prevalence of internalisation-associated gene, prtF1, among persisting group-A streptococcus strains isolated from asymptomatic carriers
Background. The failure of antibiotic treatment to eradicate group-A streptococci in up to 30% of patients with pharyngotonsillitis is unexplained. Some strains of group-A streptococci can enter respiratory epithelial cells, where they would be inaccessible to antibiotics unable to penetrate the cell membrane, such as penicillins. The fibronectin-binding proteins, F1 and SfbI, are needed for this process. We hypothesised, therefore, that an intracellular reservoir of group-A streptococci could account, at least partly, for failure to eradicate throat carriage, and that the presence of the gene for fibronectin-binding protein (F1) might be linked to the ability of a strain to persist in the throat after therapy. Methods. We investigated the frequency of prtF1-containing strains among 67 patients with pharyngotonsillitis. All patients were clinically cured, although 13 of them continued to carry group-A streptococci in the throat during or after therapy. To distinguish between persisting and recolonising strains, isolates from the 13 patients were serologically tested and compared by polymorphic DNA-amplification technique. Findings. 12 (92%) of the 13 patients with symptomless had prtF1-containing strains in the throat, with 16 (30%) of the 54 patients with eradication (p = 0.0001). Three of the 13 eradication-failure patients were recolonised with strains that differed from the pretreatment strains. Nine of the ten (90%) persisting strains carried prtF1 (p = 0.0009). Interpretation. Our findings suggest that protein-F1-mediated entry to cells is involved in the causative process of the carriage state.
Scientific Publication
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