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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a bovine mastitis Escherichia coli subset
Year:
2008
Source of publication :
Veterinary Microbiology
Authors :
המר-מונץ, אורלי
;
.
סלע, שלמה
;
.
Volume :
132
Co-Authors:
Blum, S., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Heller, E.D., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot, Israel
Krifucks, O., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization (ARO), Bet Dagan, Israel
Hammer-Muntz, O., Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization (ARO), Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Facilitators :
From page:
135
To page:
148
(
Total pages:
14
)
Abstract:
Eleven Escherichia coli isolates from clinical bovine mastitis cases (mastitic strains) and 11 from the cowshed environment (environmental strains) were compared, to determine if the former were a subset of the latter. The mastitic and environmental strains could not be distinguished according to O antigen and antibiotic sensitivity. All mastitic isolates showed significantly (P < 0.0001) faster growth in milk and faster lactose fermentation than most (∼64%) environmental strains, but growth rates in nutrient broth did not differ. The rates of lactose fermentation and growth in milk were positively correlated. Adhesion and phagocytosis of mastitic strains by bovine PMN were significantly (P < 0.0001) lower than those of environmental strains, and correlated negatively with growth in milk and lactose fermentation. The average percentages of killing by bovine leukocytes in the two sources were not statistically different. All mastitic strains were serum sensitive, whereas most (∼72%) environmental ones were resistant. Finally, pulse-field gel electrophoresis revealed two main pulse type clusters, sharing a similarity coefficient of 79%. Cluster 1 comprised only environmental strains, whereas cluster 2 comprised mostly mastitic strains and only three environmental ones. Four mastitic strains shared a similarity coefficient of less than 74% with the other strains and were not included in the clusters. Our results suggest that clinical bovine mastitis E. coli isolates may form a subset of the general environmental E. coli population; they seem better able to multiply in the udder medium and to evade the host cellular innate immune response, and are genetically distinct from most environmental strains. © 2008 Elsevier B.V. All rights reserved.
Note:
Related Files :
Animals
bacterium identification
cattle
Female
Mastitis, Bovine
milk
Neutrophils
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.vetmic.2008.05.012
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18400
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
You may also be interested in
Scientific Publication
Identification of a bovine mastitis Escherichia coli subset
132
Blum, S., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Heller, E.D., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, Rehovot, Israel
Krifucks, O., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization (ARO), Bet Dagan, Israel
Hammer-Muntz, O., Microbial Food-Safety Research Unit, Department of Food Science, Agricultural Research Organization (ARO), Bet Dagan, Israel
Leitner, G., National Mastitis Reference Center, Division of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel
Identification of a bovine mastitis Escherichia coli subset
Eleven Escherichia coli isolates from clinical bovine mastitis cases (mastitic strains) and 11 from the cowshed environment (environmental strains) were compared, to determine if the former were a subset of the latter. The mastitic and environmental strains could not be distinguished according to O antigen and antibiotic sensitivity. All mastitic isolates showed significantly (P < 0.0001) faster growth in milk and faster lactose fermentation than most (∼64%) environmental strains, but growth rates in nutrient broth did not differ. The rates of lactose fermentation and growth in milk were positively correlated. Adhesion and phagocytosis of mastitic strains by bovine PMN were significantly (P < 0.0001) lower than those of environmental strains, and correlated negatively with growth in milk and lactose fermentation. The average percentages of killing by bovine leukocytes in the two sources were not statistically different. All mastitic strains were serum sensitive, whereas most (∼72%) environmental ones were resistant. Finally, pulse-field gel electrophoresis revealed two main pulse type clusters, sharing a similarity coefficient of 79%. Cluster 1 comprised only environmental strains, whereas cluster 2 comprised mostly mastitic strains and only three environmental ones. Four mastitic strains shared a similarity coefficient of less than 74% with the other strains and were not included in the clusters. Our results suggest that clinical bovine mastitis E. coli isolates may form a subset of the general environmental E. coli population; they seem better able to multiply in the udder medium and to evade the host cellular innate immune response, and are genetically distinct from most environmental strains. © 2008 Elsevier B.V. All rights reserved.
Scientific Publication
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