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פותח על ידי קלירמאש פתרונות בע"מ -
Isolation of polymorphic AGC repeats located 3‘ to bovine SINEs
Year:
1994
Source of publication :
Animal Genetics
Authors :
רון, מיכה
;
.
Volume :
25
Co-Authors:
Band, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, 50250, Israel
Ron, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
281
To page:
283
(
Total pages:
3
)
Abstract:
A bovine genomic library was screened for the presence of (AGC)n repeats. All isolated AGC repeats were located adjacent to the 3′ end of bovine short interspersed nuclear elements (SINE). Polymerase chain reactions (PCR) using either two unique primers or one unique and one SINE primer produced high‐resolution products without the secondary artifact ladders typical of dinucleotide microsatellites. Four AGC microsatellites were found to be polymorphic with 2–4 alleles each and polymorphism information context (PIC) values ranging between 0.26 and 0.49. One microsatellite, AR025, was mapped to chromosome 26 with the CSIRO reference families. Because of their strong association with AGC repeats and high frequency in the genome, SINE‐3′ PCR may prove to be a novel source of polymorphic trinucleotide markers in the bovine genome. © 1994 International Society for Animal Genetics
Note:
Related Files :
Animal
Base Sequence
cattle
Female
Genetics
Male
molecular genetics
Repetitive Sequences, Nucleic Acid
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-2052.1994.tb00206.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18409
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
Scientific Publication
Isolation of polymorphic AGC repeats located 3‘ to bovine SINEs
25
Band, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, 50250, Israel
Ron, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, 50250, Israel
Isolation of polymorphic AGC repeats located 3‘ to bovine SINEs
A bovine genomic library was screened for the presence of (AGC)n repeats. All isolated AGC repeats were located adjacent to the 3′ end of bovine short interspersed nuclear elements (SINE). Polymerase chain reactions (PCR) using either two unique primers or one unique and one SINE primer produced high‐resolution products without the secondary artifact ladders typical of dinucleotide microsatellites. Four AGC microsatellites were found to be polymorphic with 2–4 alleles each and polymorphism information context (PIC) values ranging between 0.26 and 0.49. One microsatellite, AR025, was mapped to chromosome 26 with the CSIRO reference families. Because of their strong association with AGC repeats and high frequency in the genome, SINE‐3′ PCR may prove to be a novel source of polymorphic trinucleotide markers in the bovine genome. © 1994 International Society for Animal Genetics
Scientific Publication
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