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FEBS Letters
Liesack, W., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Sela, S., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Pitulle, C., Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universitẗt, 2300 Kiel, Germany
Stackebrandt, E., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
The complete nucleotide sequences of the Mycobacterium leprae 23 S and 5 S rRNA genes and their flanking regions are presented. As compared to other eubacterial homologous molecules the 23 S rDNA exhibits two insertions. A 16 nucleotide long insertion is almost unique to members of the genus Mycobacterium, while the second represents an extended version of helix 54. The potential or both insertions to serve as target for diagnostic oligonucleotide probes was proven by comparative sequence analysis of 23 S rRNA or several Mycobacterium species and by dot blot hybridization. In addition, a 19-mer oligonucleotide probe is described, which can be considered genus Mycobacterium-specific. © 1991.
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Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes
281
Liesack, W., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Sela, S., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Pitulle, C., Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universitẗt, 2300 Kiel, Germany
Stackebrandt, E., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes
The complete nucleotide sequences of the Mycobacterium leprae 23 S and 5 S rRNA genes and their flanking regions are presented. As compared to other eubacterial homologous molecules the 23 S rDNA exhibits two insertions. A 16 nucleotide long insertion is almost unique to members of the genus Mycobacterium, while the second represents an extended version of helix 54. The potential or both insertions to serve as target for diagnostic oligonucleotide probes was proven by comparative sequence analysis of 23 S rRNA or several Mycobacterium species and by dot blot hybridization. In addition, a 19-mer oligonucleotide probe is described, which can be considered genus Mycobacterium-specific. © 1991.
Scientific Publication
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