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פותח על ידי קלירמאש פתרונות בע"מ -
Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes
Year:
1991
Source of publication :
FEBS Letters
Authors :
סלע, שלמה
;
.
Volume :
281
Co-Authors:
Liesack, W., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Sela, S., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Pitulle, C., Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universitẗt, 2300 Kiel, Germany
Stackebrandt, E., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Facilitators :
From page:
114
To page:
118
(
Total pages:
5
)
Abstract:
The complete nucleotide sequences of the Mycobacterium leprae 23 S and 5 S rRNA genes and their flanking regions are presented. As compared to other eubacterial homologous molecules the 23 S rDNA exhibits two insertions. A 16 nucleotide long insertion is almost unique to members of the genus Mycobacterium, while the second represents an extended version of helix 54. The potential or both insertions to serve as target for diagnostic oligonucleotide probes was proven by comparative sequence analysis of 23 S rRNA or several Mycobacterium species and by dot blot hybridization. In addition, a 19-mer oligonucleotide probe is described, which can be considered genus Mycobacterium-specific. © 1991.
Note:
Related Files :
Base Sequence
diagnosis
DNA, Ribosomal
Molecular Sequence Data
Oligonucleotide Probes
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0014-5793(91)80372-A
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18430
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
Scientific Publication
Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes
281
Liesack, W., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Sela, S., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University, Medical School, Jerusalem, 91010, Israel
Pitulle, C., Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universitẗt, 2300 Kiel, Germany
Stackebrandt, E., Department of Microbiology, University of Queensland, St. Lucia, 4072, Australia
Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes
The complete nucleotide sequences of the Mycobacterium leprae 23 S and 5 S rRNA genes and their flanking regions are presented. As compared to other eubacterial homologous molecules the 23 S rDNA exhibits two insertions. A 16 nucleotide long insertion is almost unique to members of the genus Mycobacterium, while the second represents an extended version of helix 54. The potential or both insertions to serve as target for diagnostic oligonucleotide probes was proven by comparative sequence analysis of 23 S rRNA or several Mycobacterium species and by dot blot hybridization. In addition, a 19-mer oligonucleotide probe is described, which can be considered genus Mycobacterium-specific. © 1991.
Scientific Publication
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