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פותח על ידי קלירמאש פתרונות בע"מ -
DNA-microarrays identification of Streptococcus mutans genes associated with biofilm thickness - 2008
Year:
2008
Source of publication :
BMC Microbiology
Authors :
שמש, משה
;
.
Volume :
8
Co-Authors:
Shemesh, M., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Tam, A., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Kott-Gutkowski, M., Microarray Service Laboratory, Medical School, Hebrew University of Jerusalem, Israel
Feldman, M., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Steinberg, D., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Facilitators :
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Total pages:
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Abstract:
Background: A biofilm is a complex community of microorganisms that develop on surfaces in diverse environments. The thickness of the biofilm plays a crucial role in the physiology of the immobilized bacteria. The most cariogenic bacteria, mutans streptococci, are common inhabitants of a dental biofilm community. In this study, DNA-microarray analysis was used to identify differentially expressed genes associated with the thickness of S. mutans biofilms. Results: Comparative transcriptome analyses indicated that expression of 29 genes was differentially altered in 400- vs. 100-microns depth and 39 genes in 200- vs. 100-microns biofilms. Only 10 S. mutans genes showed differential expression in both 400- vs. 100-microns and 200- vs. 100-microns biofilms. All of these genes were upregulated. As sucrose is a predominant factor in oral biofilm development, its influence was evaluated on selected genes expression in the various depths of biofilms. The presence of sucrose did not noticeably change the regulation of these genes in 400- vs. 100-microns and/or 200- vs. 100-microns biofilms tested by real-time RT-PCR. Furthermore, we analyzed the expression profile of selected biofilm thickness associated genes in the luxS- mutant strain. The expression of those genes was not radically changed in the mutant strain compared to wild-type bacteria in planktonic condition. Only slight downregulation was recorded in SMU.2146c, SMU.574, SMU.609, and SMU.987 genes expression in luxS- bacteria in biofilm vs. planktonic environments. Conclusion: These findings reveal genes associated with the thickness of biofilms of S. mutans. Expression of these genes is apparently not regulated directly by luxS and is not necessarily influenced by the presence of sucrose in the growth media. © 2008 Shemesh et al; licensee BioMed Central Ltd.
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More details
DOI :
10.1186/1471-2180-8-236
Article number:
236
Affiliations:
Database:
סקופוס
Publication Type:
סקירה
;
.
Language:
אנגלית
Editors' remarks:
ID:
18456
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:21
Scientific Publication
DNA-microarrays identification of Streptococcus mutans genes associated with biofilm thickness - 2008
8
Shemesh, M., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Tam, A., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Kott-Gutkowski, M., Microarray Service Laboratory, Medical School, Hebrew University of Jerusalem, Israel
Feldman, M., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Steinberg, D., Biofilm Research Laboratory, Institute of Dental Sciences, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
DNA-microarrays identification of Streptococcus mutans genes associated with biofilm thickness
Background: A biofilm is a complex community of microorganisms that develop on surfaces in diverse environments. The thickness of the biofilm plays a crucial role in the physiology of the immobilized bacteria. The most cariogenic bacteria, mutans streptococci, are common inhabitants of a dental biofilm community. In this study, DNA-microarray analysis was used to identify differentially expressed genes associated with the thickness of S. mutans biofilms. Results: Comparative transcriptome analyses indicated that expression of 29 genes was differentially altered in 400- vs. 100-microns depth and 39 genes in 200- vs. 100-microns biofilms. Only 10 S. mutans genes showed differential expression in both 400- vs. 100-microns and 200- vs. 100-microns biofilms. All of these genes were upregulated. As sucrose is a predominant factor in oral biofilm development, its influence was evaluated on selected genes expression in the various depths of biofilms. The presence of sucrose did not noticeably change the regulation of these genes in 400- vs. 100-microns and/or 200- vs. 100-microns biofilms tested by real-time RT-PCR. Furthermore, we analyzed the expression profile of selected biofilm thickness associated genes in the luxS- mutant strain. The expression of those genes was not radically changed in the mutant strain compared to wild-type bacteria in planktonic condition. Only slight downregulation was recorded in SMU.2146c, SMU.574, SMU.609, and SMU.987 genes expression in luxS- bacteria in biofilm vs. planktonic environments. Conclusion: These findings reveal genes associated with the thickness of biofilms of S. mutans. Expression of these genes is apparently not regulated directly by luxS and is not necessarily influenced by the presence of sucrose in the growth media. © 2008 Shemesh et al; licensee BioMed Central Ltd.
Scientific Publication
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