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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
In vitro multiplication in liquid culture of Syngonium contaminated with Bacillus spp. and Rathayibacter tritici
Year:
1996
Authors :
אלפר, יקותיאל
;
.
ותד, עבד
;
.
סתו, רן
;
.
Volume :
45
Co-Authors:
Levin, R., Osmotek Ltd., P.O.B. 550, Rehovot, Israel
Stav, R., Department of Ornamental Horticulture, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Alper, Y., Department of Agriculture Engineering, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Watad, A.A., Department of Ornamental Horticulture, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
277
To page:
280
(
Total pages:
4
)
Abstract:
Contaminated Syngonium clusters were multiplied in an air lift bioreactor in liquid medium containing sucrose with the medium being circulated through a sterilizing filter. After 30 days, the culture in filtered medium produced 19.5 shoot initials per gram fresh weight of inoculum compared to 8.7 shoot initials produced in unfiltered medium. Transfer to an elongation medium with 30 mg l-1 Rifampicin produced shoots on 67% of the clusters, while transfer to elongation medium without Rifampicin produced shoots on 40% of the clusters. Clusters grown for three subcultures in a reactor without medium filtration had lost their multiplication ability. Clusters grown for three subcultures in a reactor with filtration, however, continued to show a two-three fold increase in fresh weight and shoot production.
Note:
Related Files :
Bioreactor
Contamination
medium filtration
micropropagation
Rathayibacter tritici
Syngonium
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18479
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:22
You may also be interested in
Scientific Publication
In vitro multiplication in liquid culture of Syngonium contaminated with Bacillus spp. and Rathayibacter tritici
45
Levin, R., Osmotek Ltd., P.O.B. 550, Rehovot, Israel
Stav, R., Department of Ornamental Horticulture, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Alper, Y., Department of Agriculture Engineering, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Watad, A.A., Department of Ornamental Horticulture, ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
In vitro multiplication in liquid culture of Syngonium contaminated with Bacillus spp. and Rathayibacter tritici
Contaminated Syngonium clusters were multiplied in an air lift bioreactor in liquid medium containing sucrose with the medium being circulated through a sterilizing filter. After 30 days, the culture in filtered medium produced 19.5 shoot initials per gram fresh weight of inoculum compared to 8.7 shoot initials produced in unfiltered medium. Transfer to an elongation medium with 30 mg l-1 Rifampicin produced shoots on 67% of the clusters, while transfer to elongation medium without Rifampicin produced shoots on 40% of the clusters. Clusters grown for three subcultures in a reactor without medium filtration had lost their multiplication ability. Clusters grown for three subcultures in a reactor with filtration, however, continued to show a two-three fold increase in fresh weight and shoot production.
Scientific Publication
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