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Plant Cell Reports
Watad, A.A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P. O. Box 6, Bet-Dagan 50250, Israel
Yun, D.-J., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Matsumoto, T., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Niu, X., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Wu, Y., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Kononowicz, A.K., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Bressan, R.A., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Hasegawa, P.M., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
We have obtained transgenic lily (Lilium longiflorum) plants after microprojectile bombardment, using the Biolistics PDS 1000/He system, of morphogenic calli derived from bulblet scales, followed by bialaphos selection. Parameters which gave the highest transient uidA expression were used: a bombardment pressure of 1100 psi, a target distance of 6 cm and a 48-h preculture on medium with 3% sucrose. A total of 1800 morphogenic calli were co-bombarded with plasmids containing either the uidA reporter or PA Tselectable marker genes. After bombardment, the calli were exposed to 2 mg/l bialaphos. Only 72 of the shoot-forming calli (4%) survived. The 72 shoot clusters produced 342 shoots on elongation medium containing 0.5 mg/l bialaphos. Only 55 plantlets survived subsequent exposure to 2.0 mg/l bialaphos. PCR analysis indicated that 19 of these plantlets contained the PAT transgene. Southern analysis of 3 of the plants indicated that all contained the PAT gene.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Microprojectile bombardment-mediated transformation of Lilium longiflorum
17
Watad, A.A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P. O. Box 6, Bet-Dagan 50250, Israel
Yun, D.-J., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Matsumoto, T., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Niu, X., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Wu, Y., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Kononowicz, A.K., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Bressan, R.A., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Hasegawa, P.M., Department of Horticulture, Purdue University, West Lafayette, IN 47907, United States
Microprojectile bombardment-mediated transformation of Lilium longiflorum
We have obtained transgenic lily (Lilium longiflorum) plants after microprojectile bombardment, using the Biolistics PDS 1000/He system, of morphogenic calli derived from bulblet scales, followed by bialaphos selection. Parameters which gave the highest transient uidA expression were used: a bombardment pressure of 1100 psi, a target distance of 6 cm and a 48-h preculture on medium with 3% sucrose. A total of 1800 morphogenic calli were co-bombarded with plasmids containing either the uidA reporter or PA Tselectable marker genes. After bombardment, the calli were exposed to 2 mg/l bialaphos. Only 72 of the shoot-forming calli (4%) survived. The 72 shoot clusters produced 342 shoots on elongation medium containing 0.5 mg/l bialaphos. Only 55 plantlets survived subsequent exposure to 2.0 mg/l bialaphos. PCR analysis indicated that 19 of these plantlets contained the PAT transgene. Southern analysis of 3 of the plants indicated that all contained the PAT gene.
Scientific Publication
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