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פותח על ידי קלירמאש פתרונות בע"מ -
Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins
Year:
1992
Source of publication :
Planta
Authors :
ורדי, עליזה
;
.
Volume :
186
Co-Authors:
Gavish, H., Department of Plant Genetics, Weizmann Institute of Science, P.O.B. 26, Rehovot, 76100, Israel
Vardi, A., Institute of Horticulture, Volcani Center, Bet-Dagan, 50250, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, P.O.B. 26, Rehovot, 76100, Israel
Facilitators :
From page:
511
To page:
517
(
Total pages:
7
)
Abstract:
Nucellar-derived cell cultures of sour orange (Citrus aurantium L.) proliferate as proembryogenic masses. By a change in the carbon source of the medium from sucrose to glycerol they are induced to undergo synchronous embryogenesis forming embryo initials that develop into globular embryos. The proembryogenic masses released glycoproteins to the medium. Exogenous addition of the glycoproteins to cells in glycerol-containing medium modified the course of embryo development in a dose-dependent manner. Addition of 20 μg · ml-1 of glycoproteins blocked embryogenesis and resulted in an accumulation of embryo initials. When glycoproteins were added to cultures containing advanced globularstage embryos further development was suppressed. The inhibitory component of the glycoproteins was found to be a family of polypeptides with apparent molecular masses of 53-57 kDa. While these proteins normally accumulated only in cultures of proembryogenic masses, they could be induced to accumulate in glycerol-containing medium by the addition of the glycoproteins. Thus, their accumulation was not a direct consequence of the type of growth medium used or the developmental state of the cultures. The results indicate that the 53-to 57 kDa glycoproteins could play a regulatory role in in-vitro embryogenesis in sour orange. The normal progression of embryo development appears to depend, in an obligatory manner, on the absence of these glycosylated extracellular proteins from the medium. © 1992 Springer-Verlag.
Note:
Related Files :
Cell culture (embryogenesis)
Citrus (embryogenesis)
glycoprotein
Protein, extracellular
Somatic embryogenesis
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00198030
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18513
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:22
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Scientific Publication
Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins
186
Gavish, H., Department of Plant Genetics, Weizmann Institute of Science, P.O.B. 26, Rehovot, 76100, Israel
Vardi, A., Institute of Horticulture, Volcani Center, Bet-Dagan, 50250, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, P.O.B. 26, Rehovot, 76100, Israel
Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins
Nucellar-derived cell cultures of sour orange (Citrus aurantium L.) proliferate as proembryogenic masses. By a change in the carbon source of the medium from sucrose to glycerol they are induced to undergo synchronous embryogenesis forming embryo initials that develop into globular embryos. The proembryogenic masses released glycoproteins to the medium. Exogenous addition of the glycoproteins to cells in glycerol-containing medium modified the course of embryo development in a dose-dependent manner. Addition of 20 μg · ml-1 of glycoproteins blocked embryogenesis and resulted in an accumulation of embryo initials. When glycoproteins were added to cultures containing advanced globularstage embryos further development was suppressed. The inhibitory component of the glycoproteins was found to be a family of polypeptides with apparent molecular masses of 53-57 kDa. While these proteins normally accumulated only in cultures of proembryogenic masses, they could be induced to accumulate in glycerol-containing medium by the addition of the glycoproteins. Thus, their accumulation was not a direct consequence of the type of growth medium used or the developmental state of the cultures. The results indicate that the 53-to 57 kDa glycoproteins could play a regulatory role in in-vitro embryogenesis in sour orange. The normal progression of embryo development appears to depend, in an obligatory manner, on the absence of these glycosylated extracellular proteins from the medium. © 1992 Springer-Verlag.
Scientific Publication
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