נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene.
Year:
1992
Source of publication :
The New biologist
Authors :
דקל, יצחק
;
.
מגל, יונת
;
.
שני, משה
;
.
Volume :
4
Co-Authors:
Dekel, I., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Magal, Y., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Pearson-White, S., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Emerson, C.P., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Shani, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Facilitators :
From page:
217
To page:
224
(
Total pages:
8
)
Abstract:
A variety of differentiated cell types can be converted to skeletal muscle cells following transfection with the myogenic regulatory gene MyoD1. To determine whether multipotent embryonic stem (ES) cells respond similarly, cultures of two ES cell lines were electroporated with a MyoD1 cDNA driven by the beta-actin promoter. All transfected clones, carrying a single copy of the exogenous gene, expressed high levels of MyoD1 mRNA. Surprisingly, although maintained in mitogen-rich medium, this ectopic expression was associated with a transactivation of the endogenous myogenin and myosin light chain 2 gene but not the endogenous MyoD1, MRF4, Myf5, the skeletal muscle actin, or the myosin heavy chain genes. Preferential myogenesis and the appearance of contracting skeletal muscle fibers were observed only when the transfected cells were allowed to differentiate in vitro, via embryoid bodies, in low-mitogen-containing medium. Myogenesis was associated with the activation of MRF4 and Myf5 genes and resulted in a significant increase in the level of myogenin mRNA. Not all cells were converted to skeletal muscle cells, indicating that only a subset of stem cells can respond to MyoD1. Moreover, the continued expression of the introduced gene was not required for myogenesis. These results show that ES cells can respond to MyoD1, but environmental factors control the expression of its myogenic differentiation function, that MyoD1 functions in ES cells even under environmental conditions that favor differentiation is not dominant (incomplete penetrance), that MyoD1 expression is required for the establishment of the myogenic program but not for its maintenance, and that the exogenous MyoD1 gene can trans-activate the endogenous myogenin and MLC2 genes in undifferentiated ES cells.
Note:
Related Files :
Animal
cell line
Genetics
Nuclear Proteins
phosphoprotein
Phosphoproteins
RNA, Messenger
Stem cell
stem cells
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18536
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:22
Scientific Publication
Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene.
4
Dekel, I., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Magal, Y., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Pearson-White, S., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Emerson, C.P., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Shani, M., Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Conditional conversion of ES cells to skeletal muscle by an exogenous MyoD1 gene.
A variety of differentiated cell types can be converted to skeletal muscle cells following transfection with the myogenic regulatory gene MyoD1. To determine whether multipotent embryonic stem (ES) cells respond similarly, cultures of two ES cell lines were electroporated with a MyoD1 cDNA driven by the beta-actin promoter. All transfected clones, carrying a single copy of the exogenous gene, expressed high levels of MyoD1 mRNA. Surprisingly, although maintained in mitogen-rich medium, this ectopic expression was associated with a transactivation of the endogenous myogenin and myosin light chain 2 gene but not the endogenous MyoD1, MRF4, Myf5, the skeletal muscle actin, or the myosin heavy chain genes. Preferential myogenesis and the appearance of contracting skeletal muscle fibers were observed only when the transfected cells were allowed to differentiate in vitro, via embryoid bodies, in low-mitogen-containing medium. Myogenesis was associated with the activation of MRF4 and Myf5 genes and resulted in a significant increase in the level of myogenin mRNA. Not all cells were converted to skeletal muscle cells, indicating that only a subset of stem cells can respond to MyoD1. Moreover, the continued expression of the introduced gene was not required for myogenesis. These results show that ES cells can respond to MyoD1, but environmental factors control the expression of its myogenic differentiation function, that MyoD1 functions in ES cells even under environmental conditions that favor differentiation is not dominant (incomplete penetrance), that MyoD1 expression is required for the establishment of the myogenic program but not for its maintenance, and that the exogenous MyoD1 gene can trans-activate the endogenous myogenin and MLC2 genes in undifferentiated ES cells.
Scientific Publication
You may also be interested in