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פותח על ידי קלירמאש פתרונות בע"מ -
Thermal denaturation of nucleic acids in polyacrylamide gels
Year:
1974
Source of publication :
Analytical Biochemistry
Authors :
רוזנר, אריה
;
.
Volume :
58
Co-Authors:
Rosner, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Edelman, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
602
To page:
608
(
Total pages:
7
)
Abstract:
A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies. Thermal denaturation profiles of DNA and ribosomal RNA in the gel were determined using a specially constructed Gel Carriage to position the appropriate fraction during spectrophotometric measurements. These profiles were compared with denaturation patterns obtained by classical methods in free solution; the two methods yielded similar patterns. Thermal denaturation profiles were also obtained for chloroplast light ribosomal RNA resolved by gel electrophoresis of total plant nucleic acids. Thus, denaturation patterns of individual, minor components present in complex nucleic acid mixtures can be directly measured in gels. © 1974.
Note:
Related Files :
DNA
Methods
nucleic acid
Plants
RNA
temperature
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0003-2697(74)90229-2
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18641
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:23
Scientific Publication
Thermal denaturation of nucleic acids in polyacrylamide gels
58
Rosner, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Edelman, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Thermal denaturation of nucleic acids in polyacrylamide gels
A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies. Thermal denaturation profiles of DNA and ribosomal RNA in the gel were determined using a specially constructed Gel Carriage to position the appropriate fraction during spectrophotometric measurements. These profiles were compared with denaturation patterns obtained by classical methods in free solution; the two methods yielded similar patterns. Thermal denaturation profiles were also obtained for chloroplast light ribosomal RNA resolved by gel electrophoresis of total plant nucleic acids. Thus, denaturation patterns of individual, minor components present in complex nucleic acid mixtures can be directly measured in gels. © 1974.
Scientific Publication
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