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The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Year:
2009
Source of publication :
Development
Authors :
ברנד, ארנון
;
.
Volume :
136
Co-Authors:
Berger, Y., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Harpaz-Saad, S., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Brand, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Melnik, H., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Sirding, N., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Alvarez, J.P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Zinder, M., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Samach, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Eshed, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Ori, N., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
823
To page:
832
(
Total pages:
10
)
Abstract:
Leaves are formed at the flanks of the shoot apical meristem (SAM) and develop into a variety of forms. In tomato, prolonged leaf patterning enables the elaboration of compound leaves by reiterative initiation of leaflets with lobed margins. In goblet (gob) loss-of-function mutants, primary leaflets are often fused, secondary leaflets and marginal serrations are absent, and SAMs often terminate precociously. We show that GOB encodes a NAC-domain transcription factor expressed in narrow stripes at the leaf margins, flanking the distal side of future leaflet primordia, and at the boundaries between the SAM and leaf primordia. Leaf-specific overexpression of the microRNA miR164, a negative regulator of GOB-like genes, also leads to loss of secondary-leaflet initiation and to smooth leaflet margins. Plants carrying a dominant gob allele with an intact ORF but disrupted miR164 binding site produce more cotyledons and floral organs, have split SAMs and, surprisingly, simpler leaves. Overexpression of a form of GOB with an altered miR164 binding site in leaf primordia leads to delayed leaflet maturation, frequent, improperly timed and spaced initiation events, and a simple mature leaflet form owing to secondary-leaflet fusion. miR164 also affects leaflet separation in Cardamine hirsuta, a Brassicaceae species with complex leaves. Genetic and molecular analyses suggest that GOB expression is intact in the simplified leaves of entire tomato mutants, which have a defect in a putative repressor of auxin responses. Our results show that GOB marks leaflet boundaries and that its accurate spatial, temporal and quantitative activity affects leaf elaboration in a context-dependent manner.
Note:
Related Files :
Brassicaceae
Cotyledon
Genetics
Growth, Development and Aging
meristem
metabolism
mutation
phenotype
plant development
עוד תגיות
תוכן קשור
More details
DOI :
10.1242/dev.031625
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18764
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
You may also be interested in
Scientific Publication
The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
136
Berger, Y., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Harpaz-Saad, S., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Brand, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Melnik, H., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Sirding, N., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Alvarez, J.P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Zinder, M., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Samach, A., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Eshed, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Ori, N., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Leaves are formed at the flanks of the shoot apical meristem (SAM) and develop into a variety of forms. In tomato, prolonged leaf patterning enables the elaboration of compound leaves by reiterative initiation of leaflets with lobed margins. In goblet (gob) loss-of-function mutants, primary leaflets are often fused, secondary leaflets and marginal serrations are absent, and SAMs often terminate precociously. We show that GOB encodes a NAC-domain transcription factor expressed in narrow stripes at the leaf margins, flanking the distal side of future leaflet primordia, and at the boundaries between the SAM and leaf primordia. Leaf-specific overexpression of the microRNA miR164, a negative regulator of GOB-like genes, also leads to loss of secondary-leaflet initiation and to smooth leaflet margins. Plants carrying a dominant gob allele with an intact ORF but disrupted miR164 binding site produce more cotyledons and floral organs, have split SAMs and, surprisingly, simpler leaves. Overexpression of a form of GOB with an altered miR164 binding site in leaf primordia leads to delayed leaflet maturation, frequent, improperly timed and spaced initiation events, and a simple mature leaflet form owing to secondary-leaflet fusion. miR164 also affects leaflet separation in Cardamine hirsuta, a Brassicaceae species with complex leaves. Genetic and molecular analyses suggest that GOB expression is intact in the simplified leaves of entire tomato mutants, which have a defect in a putative repressor of auxin responses. Our results show that GOB marks leaflet boundaries and that its accurate spatial, temporal and quantitative activity affects leaf elaboration in a context-dependent manner.
Scientific Publication
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