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Colletotrichum gloeosporioides pelB is an important virulence factor in avocado fruit-fungus interaction
Year:
2001
Authors :
בנו-מועלם, דלילה
;
.
פרוסקי, דב
;
.
Volume :
14
Co-Authors:
Yakoby, N., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Beno-Moualem, D., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Keen, N.T., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pines, O., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
988
To page:
995
(
Total pages:
8
)
Abstract:
Colletotrichum gloeosporioides is an important pathogen of tropical and subtropical fruits. The C. gloeosporioides pelB gene was disrupted in the fungus via homologous recombination. Three independent isolates, GD-14, GD-23, and GD-29, did not produce or secrete pectate lyase B (PLB) and exhibited 25% lower pectate lyase (PL) and pectin lyase (PNL) activities and 15% higher polygalacturonase (PG) activity than the wild type. The PLB mutants exhibited no growth reduction on glucose, Na polypectate, or pectin as the sole carbon source at pH 3.8 or 6.0, except for a 15% reduction on pectin at pH 6.0. When pelB mutants were inoculated onto avocado fruits, however, a 36 to 45% reduction in estimated decay diameter was observed compared with the two controls, the wild type and undisrupted transformed isolate. In addition, these pelB mutants induced a significantly higher host phenylalanine ammonia lyase activity as well as the antifungal diene, which is indicative of higher host resistance. These results suggest that PLB is an important factor in the attack of C. gloeosporioides on avocado fruit, probably as a result of its virulence factor and role in the induction of host defense mechanisms.
Note:
Related Files :
Colletotrichum
enzyme activity
fungi
host resistance
Inoculation
Persea americana
pH
Plant Diseases
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18773
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
Colletotrichum gloeosporioides pelB is an important virulence factor in avocado fruit-fungus interaction
14
Yakoby, N., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Beno-Moualem, D., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Keen, N.T., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pines, O., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Prusky, D., Dept. Postharvest Sci. Fresh Prod., Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Colletotrichum gloeosporioides pelB is an important virulence factor in avocado fruit-fungus interaction
Colletotrichum gloeosporioides is an important pathogen of tropical and subtropical fruits. The C. gloeosporioides pelB gene was disrupted in the fungus via homologous recombination. Three independent isolates, GD-14, GD-23, and GD-29, did not produce or secrete pectate lyase B (PLB) and exhibited 25% lower pectate lyase (PL) and pectin lyase (PNL) activities and 15% higher polygalacturonase (PG) activity than the wild type. The PLB mutants exhibited no growth reduction on glucose, Na polypectate, or pectin as the sole carbon source at pH 3.8 or 6.0, except for a 15% reduction on pectin at pH 6.0. When pelB mutants were inoculated onto avocado fruits, however, a 36 to 45% reduction in estimated decay diameter was observed compared with the two controls, the wild type and undisrupted transformed isolate. In addition, these pelB mutants induced a significantly higher host phenylalanine ammonia lyase activity as well as the antifungal diene, which is indicative of higher host resistance. These results suggest that PLB is an important factor in the attack of C. gloeosporioides on avocado fruit, probably as a result of its virulence factor and role in the induction of host defense mechanisms.
Scientific Publication
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