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פותח על ידי קלירמאש פתרונות בע"מ -
A combination of glycerol and manganese promotes biofilm formation in Bacillus subtilis via histidine kinase KinD signaling
Year:
2013
Source of publication :
Journal of Bacteriology
Authors :
שמש, משה
;
.
Volume :
195
Co-Authors:
Shemesh, M., Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, United States, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan, Israel
Chaia, Y., Department of Biology, Northeastern University, Boston, MA, United States
Facilitators :
From page:
2747
To page:
2754
(
Total pages:
8
)
Abstract:
The spore-forming bacterium Bacillus subtilis forms matrix-enclosed biofilms in response to environmental cues that to date remain poorly defined. Biofilm formation depends on the synthesis of an extracellular matrix, which is indirectly regulated by the transcriptional regulator Spo0A. The activity of Spo0A depends on its phosphorylation state. The level of phosphorylated Spo0A (Spo0A~P) is controlled by a network of kinases and phosphatases, which respond to environmental and physiological signals. In spite of significant progress in understanding biofilm development, the fundamental question of how cells sense the environmental cues that trigger biofilm formation has largely remained unaddressed. Here, we report that biofilm formation of B. subtilis in LB medium is triggered by a combination of glycerol and manganese (GM). Moreover, LB medium with GM significantly stimulates biofilm-associated sporulation and production of an undefined brown pigment. We further show that transcription of the major operons responsible for matrix production and biofilm formation is dramatically enhanced in response to GM. We also establish that KinD is a principal histidine kinase responsible for sensing the presence of GM exclusively by its extracellular CACHE domain. Finally, we show that GM has a similar biofilm-promoting effect in two related Bacillus species, B. licheniformis and B. cereus, indicating that the biofilm-promoting effect of GM is conserved in Bacillus species. © 2013, American Society for Microbiology.
Note:
Related Files :
bacillus licheniformis
glycerol
histidine kinase kind
protein histidine kinase
Protein Kinases
signal transduction
unclassified drug
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More details
DOI :
10.1128/JB.00028-13
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18785
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
A combination of glycerol and manganese promotes biofilm formation in Bacillus subtilis via histidine kinase KinD signaling
195
Shemesh, M., Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, United States, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan, Israel
Chaia, Y., Department of Biology, Northeastern University, Boston, MA, United States
A combination of glycerol and manganese promotes biofilm formation in Bacillus subtilis via histidine kinase KinD signaling
The spore-forming bacterium Bacillus subtilis forms matrix-enclosed biofilms in response to environmental cues that to date remain poorly defined. Biofilm formation depends on the synthesis of an extracellular matrix, which is indirectly regulated by the transcriptional regulator Spo0A. The activity of Spo0A depends on its phosphorylation state. The level of phosphorylated Spo0A (Spo0A~P) is controlled by a network of kinases and phosphatases, which respond to environmental and physiological signals. In spite of significant progress in understanding biofilm development, the fundamental question of how cells sense the environmental cues that trigger biofilm formation has largely remained unaddressed. Here, we report that biofilm formation of B. subtilis in LB medium is triggered by a combination of glycerol and manganese (GM). Moreover, LB medium with GM significantly stimulates biofilm-associated sporulation and production of an undefined brown pigment. We further show that transcription of the major operons responsible for matrix production and biofilm formation is dramatically enhanced in response to GM. We also establish that KinD is a principal histidine kinase responsible for sensing the presence of GM exclusively by its extracellular CACHE domain. Finally, we show that GM has a similar biofilm-promoting effect in two related Bacillus species, B. licheniformis and B. cereus, indicating that the biofilm-promoting effect of GM is conserved in Bacillus species. © 2013, American Society for Microbiology.
Scientific Publication
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