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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles
Year:
1987
Source of publication :
Theoretical and Applied Genetics
Authors :
ורדי, עליזה
;
.
Volume :
75
Co-Authors:
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Breiman, A., Department of Botany, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
51
To page:
58
(
Total pages:
8
)
Abstract:
Embryogenic nucellar-callus from three Citrus types was used for protoplast isolation. The protoplasts were fused by the 'donor-recipient' procedure by which the nuclear-division of the donor-protoplasts was arrested by gamma-irradiation and the metabolism of unfused recipient-protoplasts was transiently inhibited by iodoacetate. The following fusion combinations were performed: (1) Poorman x Poncirus trifoliata with Villafranca lemon; (2) Poorman x P. trifoliata with Sour orange; (3) Sour orange with Villafranca. Combinations 1, 2 and 3 resulted in 120 (125), 70 (5) and 19 (89) calli (regenerated plants), respectively. The mitochondrial-DNA (mtDNA) restriction profiles of Poorman x P. trifoliata obtained by fragmentation with Bcl I, Bam H1 or Sal I differed from the respective profiles of Villafranca and Sour orange but no differences in mtDNA restriction profiles were detected between Villafranca and Sour orange. When Southern blots of Villafranca and Sour orange mtDNAs were hybridized with radiolabelled heterologous mtDNA probes, the mtDNAs of these two Citrus types could be differentiated. The fusion-derived plants from all three donor-recipient combinations had the recipients morphological (nuclear-coded) features. MtDNA restriction profiles, with and without hybridization to heterologous probes, indicated that the analysed plants were cybrids. © 1987 Springer-Verlag.
Note:
Related Files :
Citrus
Cybrids
mitochondrial DNA
Protoplasts
Southern blot hybridization
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00249142
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18826
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles
75
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Breiman, A., Department of Botany, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles
Embryogenic nucellar-callus from three Citrus types was used for protoplast isolation. The protoplasts were fused by the 'donor-recipient' procedure by which the nuclear-division of the donor-protoplasts was arrested by gamma-irradiation and the metabolism of unfused recipient-protoplasts was transiently inhibited by iodoacetate. The following fusion combinations were performed: (1) Poorman x Poncirus trifoliata with Villafranca lemon; (2) Poorman x P. trifoliata with Sour orange; (3) Sour orange with Villafranca. Combinations 1, 2 and 3 resulted in 120 (125), 70 (5) and 19 (89) calli (regenerated plants), respectively. The mitochondrial-DNA (mtDNA) restriction profiles of Poorman x P. trifoliata obtained by fragmentation with Bcl I, Bam H1 or Sal I differed from the respective profiles of Villafranca and Sour orange but no differences in mtDNA restriction profiles were detected between Villafranca and Sour orange. When Southern blots of Villafranca and Sour orange mtDNAs were hybridized with radiolabelled heterologous mtDNA probes, the mtDNAs of these two Citrus types could be differentiated. The fusion-derived plants from all three donor-recipient combinations had the recipients morphological (nuclear-coded) features. MtDNA restriction profiles, with and without hybridization to heterologous probes, indicated that the analysed plants were cybrids. © 1987 Springer-Verlag.
Scientific Publication
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