נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Heterologous reactivity of tobacco mosaic virus strains in enzyme-linked immunosorbent assays
Year:
1980
Source of publication :
Journal of General Virology
Authors :
בר-יוסף, משה
;
.
סלומון, רפי
;
.
Volume :
47
Co-Authors:
Bar-Joseph, M., Agric. Res. Organ., Volcani Cent., Bet Dagan, Israel
Salomon, R., Agric. Res. Organ., Volcani Cent., Bet Dagan, Israel
Facilitators :
From page:
509
To page:
512
(
Total pages:
4
)
Abstract:
Enzyme-linked immunosorbent assay (ELISA) differentiated between the antigens of the type strains of tobacco mosaic virus (TMV) and those of the avocado isolate (TMV-A). The ELISA specificity in the heterologous antibody systems was affected mainly by the behaviour of the free phase (conjugate antibodies) whereas antibodies used for coating cross-reacted as in double-diffusion tests. Binding of bovine serum albumin to the TMV and TMV-A antibodies used for coating impaired their activity when tested in homologous reactions but did not make them completely specific in the heterologous system. The complexes of both TMV and TMV-A antigens bound to their homologous and heterologous coated antibodies were stable to acid dissociation. Some possible explanations of ELISA specificity are discussed.
Note:
Related Files :
enzyme immunoassay
higher plant
in vitro study
Tobacco mosaic virus
virus strain
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
Language:
אנגלית
Editors' remarks:
ID:
18860
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
Scientific Publication
Heterologous reactivity of tobacco mosaic virus strains in enzyme-linked immunosorbent assays
47
Bar-Joseph, M., Agric. Res. Organ., Volcani Cent., Bet Dagan, Israel
Salomon, R., Agric. Res. Organ., Volcani Cent., Bet Dagan, Israel
Heterologous reactivity of tobacco mosaic virus strains in enzyme-linked immunosorbent assays
Enzyme-linked immunosorbent assay (ELISA) differentiated between the antigens of the type strains of tobacco mosaic virus (TMV) and those of the avocado isolate (TMV-A). The ELISA specificity in the heterologous antibody systems was affected mainly by the behaviour of the free phase (conjugate antibodies) whereas antibodies used for coating cross-reacted as in double-diffusion tests. Binding of bovine serum albumin to the TMV and TMV-A antibodies used for coating impaired their activity when tested in homologous reactions but did not make them completely specific in the heterologous system. The complexes of both TMV and TMV-A antigens bound to their homologous and heterologous coated antibodies were stable to acid dissociation. Some possible explanations of ELISA specificity are discussed.
Scientific Publication
You may also be interested in