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הספר "אוצר וולקני"
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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Molecular mapping of the C locus for presence of pungency in Capsicum
Year:
2002
Source of publication :
Genome
Authors :
פארן, אילן
;
.
Volume :
45
Co-Authors:
Blum, E., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liu, K., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Mazourek, M., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yoo, E.Y., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Jahn, M., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Paran, I., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
702
To page:
705
(
Total pages:
4
)
Abstract:
Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus is used in breeding programs around the world focused on very diverse germplasm, hence any of these tightly linked markers may be of value as potential sources of useful markers for marker-assisted selection. To demonstrate this point, we developed a PCR-based CAPS (cleaved amplified polymorphic sequence) marker linked to C using the sequence of the Capsicum fibrillin gene located 0.4 cM from C. The use of molecular markers for high-throughput screening for the c allele in pepper breeding programs is discussed.
Note:
Related Files :
Capsicum annuum
chromosome mapping
genetic markers
Molecular Sequence Data
Polymerase Chain Reaction
Pungency
עוד תגיות
תוכן קשור
More details
DOI :
10.1139/g02-031
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18869
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
Scientific Publication
Molecular mapping of the C locus for presence of pungency in Capsicum
45
Blum, E., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liu, K., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Mazourek, M., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Yoo, E.Y., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Jahn, M., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Paran, I., Department of Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Molecular mapping of the C locus for presence of pungency in Capsicum
Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus is used in breeding programs around the world focused on very diverse germplasm, hence any of these tightly linked markers may be of value as potential sources of useful markers for marker-assisted selection. To demonstrate this point, we developed a PCR-based CAPS (cleaved amplified polymorphic sequence) marker linked to C using the sequence of the Capsicum fibrillin gene located 0.4 cM from C. The use of molecular markers for high-throughput screening for the c allele in pepper breeding programs is discussed.
Scientific Publication
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