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פותח על ידי קלירמאש פתרונות בע"מ -
Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)
Year:
2005
Source of publication :
Theoretical and Applied Genetics
Authors :
קציר, נורית
;
.
Volume :
110
Co-Authors:
Gonzalo, M.J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Oliver, M., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain, Syngenta Seeds S.A.S, 12 Chemin de l'Hobit, 31790 Saint Sauveur, France
Garcia-Mas, J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Monfort, A., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Dolcet-Sanjuan, R., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Katzir, N., Dept. of Genet. and Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay, 30-095, Israel
Arús, P., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Monforte, A.J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Facilitators :
From page:
802
To page:
811
(
Total pages:
10
)
Abstract:
A set of 118 simple sequence repeat (SSR) markers has been developed in melon from two different sources: genomic libraries (gSSR) and expressed sequence-tag (EST) databases (EST-SSR). Forty-nine percent of the markers showed polymorphism between the 'Piel de Sapo' (PS) and PI161375 melon genotypes used as parents for the mapping populations. Similar polymorphism levels were found in gSSR (51.2%) and EST-SSR (45.5%). Two populations, F2 and a set of double haploid lines (DHLs), developed from the same parent genotypes were used for map construction. Twenty-three SSRs and 79 restriction fragment length polymorphisms (RFLPs), evenly distributed through the melon genome, were used to anchor the maps of both populations. Ten cucumber SSRs, 41 gSSRs, 16 EST-SSR, three single nucleotide polymorphism (SNP) markers, and the Nsv locus were added in the DHL population. The maps developed in the F2 and DHL populations were co-linear, with similar lengths, except in linkage groups G1, G9, and G10. There was segregation distortion in a higher proportion of markers in the DHL population compared with the F2, probably caused by selection during the construction of DHLs through in vitro culture. After map merging, a composite genetic map was obtained including 327 transferable markers: 226 RFLPs, 97 SSRs, three SNPs, and the Nsv locus. The map length is 1,021 cM, distributed in 12 linkage groups, and map density is 3.11 cM/marker. SSR markers alone cover nearly 80% of the map length. This map is proposed as a basis for a framework melon map to be merged with other maps and as an anchor point for map comparison between species of the Cucurbitaceae family. © Springer-Verlag 2005.
Note:
Related Files :
chromosome mapping
Cucumis melo
Cucumis sativus
Cucurbitaceae
Genes
Genetics
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-004-1814-6
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18872
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:24
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Scientific Publication
Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)
110
Gonzalo, M.J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Oliver, M., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain, Syngenta Seeds S.A.S, 12 Chemin de l'Hobit, 31790 Saint Sauveur, France
Garcia-Mas, J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Monfort, A., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Dolcet-Sanjuan, R., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Katzir, N., Dept. of Genet. and Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay, 30-095, Israel
Arús, P., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Monforte, A.J., Laboratori CSIC-IRTA Genet. M., Carretera Cabrils s/n, 08348 Cabrils (Barcelona), Spain
Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)
A set of 118 simple sequence repeat (SSR) markers has been developed in melon from two different sources: genomic libraries (gSSR) and expressed sequence-tag (EST) databases (EST-SSR). Forty-nine percent of the markers showed polymorphism between the 'Piel de Sapo' (PS) and PI161375 melon genotypes used as parents for the mapping populations. Similar polymorphism levels were found in gSSR (51.2%) and EST-SSR (45.5%). Two populations, F2 and a set of double haploid lines (DHLs), developed from the same parent genotypes were used for map construction. Twenty-three SSRs and 79 restriction fragment length polymorphisms (RFLPs), evenly distributed through the melon genome, were used to anchor the maps of both populations. Ten cucumber SSRs, 41 gSSRs, 16 EST-SSR, three single nucleotide polymorphism (SNP) markers, and the Nsv locus were added in the DHL population. The maps developed in the F2 and DHL populations were co-linear, with similar lengths, except in linkage groups G1, G9, and G10. There was segregation distortion in a higher proportion of markers in the DHL population compared with the F2, probably caused by selection during the construction of DHLs through in vitro culture. After map merging, a composite genetic map was obtained including 327 transferable markers: 226 RFLPs, 97 SSRs, three SNPs, and the Nsv locus. The map length is 1,021 cM, distributed in 12 linkage groups, and map density is 3.11 cM/marker. SSR markers alone cover nearly 80% of the map length. This map is proposed as a basis for a framework melon map to be merged with other maps and as an anchor point for map comparison between species of the Cucurbitaceae family. © Springer-Verlag 2005.
Scientific Publication
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