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פותח על ידי קלירמאש פתרונות בע"מ -
Pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster
Year:
2001
Source of publication :
Cell and Tissue Research
Authors :
רפאלי, עדה
;
.
Volume :
306
Co-Authors:
Choi, M.-Y., Department of Entomology, 407 Science II, Iowa State University, Ames, IA 50011-3222, United States
Rafaeli, A., Department of Stored Products, Volcani Center, ARO, P.O. Box 6, Bet Dagan 50250, Israel
Jurenka, R.A., Department of Entomology, 407 Science II, Iowa State University, Ames, IA 50011-3222, United States
Facilitators :
From page:
459
To page:
465
(
Total pages:
7
)
Abstract:
The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides found in insects is characterized by a 5-amino-acid C-terminal sequence, FXPRLamide. The pentapeptide is the active core required for diverse physiological functions, including stimulation of pheromone biosynthesis in female moths, stimulation of muscle contraction, induction of embryonic diapause in Bombyx mori, and stimulation of melanization in some larval moths. Recently, this family of peptides has been implicated in accelerating the formation of the puparium in a dipteran. Using bioassay and immunocytochemical techniques, we demonstrate the presence of pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster. Pheromonotropic activity was shown in the moths Helicoverpa zea and Helicoverpa armigera by using dissected larval nervous systems and adult heads and bodies of D. melanogaster. Polyclonal antisera against the C-terminal ending of PBAN revealed the location of cell bodies and axons in the central nervous systems of larval and adult flies. Immunoreactive material was detected in at least three groups of neurons in the subesophageal ganglion of 3rd instar larvae, pupae, and adults. The ring gland of both larvae and adults contained immunoreactivity. Adult brain-subesophageal ganglion complex possessed additional neurons. The fused ventral ganglia of both larvae and adults contained three pairs of neurons that sent their axons to a neurohemal organ connected to the abdominal nervous system. These results indicate that the D. melanogaster nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph.
Note:
Related Files :
animal experiment
Animals
animal tissue
carboxy terminal sequence
Female
ganglion
Lepidoptera
Male
neuropeptide
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00441-001-0467-x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18930
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
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Scientific Publication
Pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster
306
Choi, M.-Y., Department of Entomology, 407 Science II, Iowa State University, Ames, IA 50011-3222, United States
Rafaeli, A., Department of Stored Products, Volcani Center, ARO, P.O. Box 6, Bet Dagan 50250, Israel
Jurenka, R.A., Department of Entomology, 407 Science II, Iowa State University, Ames, IA 50011-3222, United States
Pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster
The pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family of peptides found in insects is characterized by a 5-amino-acid C-terminal sequence, FXPRLamide. The pentapeptide is the active core required for diverse physiological functions, including stimulation of pheromone biosynthesis in female moths, stimulation of muscle contraction, induction of embryonic diapause in Bombyx mori, and stimulation of melanization in some larval moths. Recently, this family of peptides has been implicated in accelerating the formation of the puparium in a dipteran. Using bioassay and immunocytochemical techniques, we demonstrate the presence of pyrokinin/PBAN-like peptides in the central nervous system of Drosophila melanogaster. Pheromonotropic activity was shown in the moths Helicoverpa zea and Helicoverpa armigera by using dissected larval nervous systems and adult heads and bodies of D. melanogaster. Polyclonal antisera against the C-terminal ending of PBAN revealed the location of cell bodies and axons in the central nervous systems of larval and adult flies. Immunoreactive material was detected in at least three groups of neurons in the subesophageal ganglion of 3rd instar larvae, pupae, and adults. The ring gland of both larvae and adults contained immunoreactivity. Adult brain-subesophageal ganglion complex possessed additional neurons. The fused ventral ganglia of both larvae and adults contained three pairs of neurons that sent their axons to a neurohemal organ connected to the abdominal nervous system. These results indicate that the D. melanogaster nervous system contains pyrokinin/PBAN-like peptides and that these peptides could be released into the hemolymph.
Scientific Publication
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