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פותח על ידי קלירמאש פתרונות בע"מ -
Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck
Year:
2001
Source of publication :
Planta
Authors :
בן חיים, גוזל
;
.
דהן, ירדנה
;
.
Volume :
212
Co-Authors:
Ben-Hayyim, G., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gueta-Dahan, Y., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Avsian-Kretchmer, O., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Weichert, H., Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany
Feussner, I., Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany
Facilitators :
From page:
367
To page:
375
(
Total pages:
9
)
Abstract:
Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic auto-oxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.
Note:
Related Files :
antioxidants
biochemical pathway
Chromatography, High Pressure Liquid
enzymic reduction
herbicides
macrogol
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s004250000397
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
18999
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
You may also be interested in
Scientific Publication
Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck
212
Ben-Hayyim, G., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gueta-Dahan, Y., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Avsian-Kretchmer, O., Department of Fruit-Tree Breeding and Molecular Genetics, ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Weichert, H., Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany
Feussner, I., Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany
Preferential induction of a 9-lipoxygenase by salt in salt-tolerant cells of Citrus sinensis L. Osbeck
Recent findings in our laboratory suggested that in citrus cells the salt induction of phospholipid hydroperoxide glutathione peroxidase, an enzyme active in cellular antioxidant defense, is mediated by the accumulation of hydroperoxides. Production of hydroperoxides occurs as a result of non-enzymatic auto-oxidation or via the action of lipoxygenases (LOXs). In an attempt to resolve the role of LOX activity in the accumulation of peroxides we analyzed the expression of this protein under stress conditions and in cells of Citrus sinensis L. differing in sensitivity to salt. Lipoxygenase expression was induced very rapidly only in the salt-tolerant cells and in a transient manner. The induction was specific to salt stress and did not occur with other osmotic-stress-inducing agents, such as polyethylene glycol or mannitol, or under hot or cold conditions, or in the presence of abscisic acid. The induction was eliminated by the antioxidants dithiothreitol and kaempferol, thus once more establishing a correlation between salt and oxidative stresses. Analyses of both in vitro and in vivo products of LOX revealed a specific 9-LOX activity, and a very fast reduction of the hydroperoxides to the corresponding hydroxy derivatives. This suggests that one of the metabolites further downstream in the reductase pathway may play a key role in triggering defense responses against salt stress.
Scientific Publication
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