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פותח על ידי קלירמאש פתרונות בע"מ -
The Tomato yellow leaf curl virus V<inf>2</inf> protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA
Year:
2015
Source of publication :
Scientific Reports
Authors :
בלאוסוב, אדוארד
;
.
Volume :
5
Co-Authors:
Moshe, A., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Bet Dagan, Israel
Niehl, A., Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France, Zürich-Basel Plant Science Center, Department of Environmental Sciences, Botany, University of Basel, Basel, Switzerland
Heinlein, M., Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France
Czosnek, H., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
Gorovits, R., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:
The spread of Tomato yellow leaf curl virus (TYLCV) was accompanied by the formation of coat protein (CP) aggregates of increasing size in the cytoplasm and nucleus of infected tomato (Solanum lycopersicum) cells. In order to better understand the TYLCV-host interaction, we investigated the properties and the subcellular accumulation pattern of the non-structural viral protein V2. CP and V2 are the only sense-oriented genes on the virus circular single-stranded DNA genome. Similar to CP, V2 localized to cytoplasmic aggregates of increasing size and as infection progressed was also found in nuclei, where it co-localized with CP. V2 was associated with viral genomic DNA molecules, suggesting that V2 functions as a DNA shuttling protein. The formation and the 26S proteasome-mediated degradation of V2 aggregates were dependent on the integrity of the actin and microtubule cytoskeleton. We propose that the cytoskeleton-dependent formation and growth of V2 aggregates play an important role during TYLCV infection, and that microtubules and actin filaments are important for the delivery of V2 to the 26S proteasome.
Note:
Related Files :
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תוכן קשור
More details
DOI :
10.1038/srep09967
Article number:
3000
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19006
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
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Scientific Publication
The Tomato yellow leaf curl virus V<inf>2</inf> protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA
5
Moshe, A., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Bet Dagan, Israel
Niehl, A., Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France, Zürich-Basel Plant Science Center, Department of Environmental Sciences, Botany, University of Basel, Basel, Switzerland
Heinlein, M., Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France
Czosnek, H., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
Gorovits, R., Institute of Plant Sciences and Genetics in Agriculture, Otto Warburg Minerva Center for Agricultural Biotechnology, Hebrew University of Jerusalem, Rehovot, Israel
The Tomato yellow leaf curl virus V<inf>2</inf> protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA
The spread of Tomato yellow leaf curl virus (TYLCV) was accompanied by the formation of coat protein (CP) aggregates of increasing size in the cytoplasm and nucleus of infected tomato (Solanum lycopersicum) cells. In order to better understand the TYLCV-host interaction, we investigated the properties and the subcellular accumulation pattern of the non-structural viral protein V2. CP and V2 are the only sense-oriented genes on the virus circular single-stranded DNA genome. Similar to CP, V2 localized to cytoplasmic aggregates of increasing size and as infection progressed was also found in nuclei, where it co-localized with CP. V2 was associated with viral genomic DNA molecules, suggesting that V2 functions as a DNA shuttling protein. The formation and the 26S proteasome-mediated degradation of V2 aggregates were dependent on the integrity of the actin and microtubule cytoskeleton. We propose that the cytoskeleton-dependent formation and growth of V2 aggregates play an important role during TYLCV infection, and that microtubules and actin filaments are important for the delivery of V2 to the 26S proteasome.
Scientific Publication
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