Co-Authors:
Chkhikvishvili, I., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Sanikidze, T., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Gogia, N., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Enukidze, M., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Machavariani, M., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Kipiani, N., Institute of Medical Biotechnology, Tbilisi State Medical University, 33 Vazha Pshavela Avenue, Tbilisi, Georgia
Vinokur, Y., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, HaMaccabim Road 68, Rishon LeZion, Israel
Rodov, V., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, HaMaccabim Road 68, Rishon LeZion, Israel
Abstract:
The flowers of French marigold (Tagetes patula L.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential of T. patula compounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential. T. patula compounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers. © 2016 Irakli Chkhikvishvili et al.
תפריט נגישות
ניגודיות עדינה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם
