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פותח על ידי קלירמאש פתרונות בע"מ -
A genomic search for the gene conferring resistance to fusarium wilt in tomato
Year:
1994
Source of publication :
Euphytica
Authors :
פארן, אילן
;
.
Volume :
79
Co-Authors:
Ori, N., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Paran, I., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Aviv, D., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Eshed, Y., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Tanksley, S., Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York, United States
Zamir, D., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
201
To page:
204
(
Total pages:
4
)
Abstract:
Fusarium wilt is an economically important disease of tomatoes, caused by the soil-born fungus Fusarium oxysporum f. sp. lycopersici. There are three host-specific races of this pathogen. The dominant tomato gene I-2 confers resistance to race 2. The I-2 fusarium resistance gene was mapped genetically to chromosome 11 of tomato, between the RFLP markers TG105 and TG36, 0.4 centiMorgan (cM) from TG105. A mean value of 43 kb for each cM was assigned in the vicinity of I-2. We have generated new RFLP markers in the region by chromosome walking from TG105 towards I-2 on lambda clones, and by subcloning a 350 kb long YAC clone (YAC 8) that contains TG105. These RFLP markers were mapped physically on YAC 8 by PFGE. The location of I-2 relative to these markers was genetically estimated using a recombinant inbred (RI) segregating population. The order of the markers according to the RI population is inconsistent with their order on the physical map. A cDNA clone, D14, that was isolated by YAC 8, turned out to be 53% similar to xanthine dehydrogenase from mammals and flies. Antibodies raised against a part of the protein encoded by D14 recognize cross reacting material of MW 80 kD, that is highly enriched in nodules of legumes, and seems to be induced by various environmental and pathogenic stress conditions. © 1994 Kluwer Academic Publishers.
Note:
Related Files :
disease resistance
i-2 positional cloning
recombinant inbreds
xanthine dehydrogenase
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00022520
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19021
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:25
You may also be interested in
Scientific Publication
A genomic search for the gene conferring resistance to fusarium wilt in tomato
79
Ori, N., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Paran, I., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Aviv, D., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Eshed, Y., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Tanksley, S., Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York, United States
Zamir, D., Faculty of Agriculture, The Hebrew University, Rehovot, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
A genomic search for the gene conferring resistance to fusarium wilt in tomato
Fusarium wilt is an economically important disease of tomatoes, caused by the soil-born fungus Fusarium oxysporum f. sp. lycopersici. There are three host-specific races of this pathogen. The dominant tomato gene I-2 confers resistance to race 2. The I-2 fusarium resistance gene was mapped genetically to chromosome 11 of tomato, between the RFLP markers TG105 and TG36, 0.4 centiMorgan (cM) from TG105. A mean value of 43 kb for each cM was assigned in the vicinity of I-2. We have generated new RFLP markers in the region by chromosome walking from TG105 towards I-2 on lambda clones, and by subcloning a 350 kb long YAC clone (YAC 8) that contains TG105. These RFLP markers were mapped physically on YAC 8 by PFGE. The location of I-2 relative to these markers was genetically estimated using a recombinant inbred (RI) segregating population. The order of the markers according to the RI population is inconsistent with their order on the physical map. A cDNA clone, D14, that was isolated by YAC 8, turned out to be 53% similar to xanthine dehydrogenase from mammals and flies. Antibodies raised against a part of the protein encoded by D14 recognize cross reacting material of MW 80 kD, that is highly enriched in nodules of legumes, and seems to be induced by various environmental and pathogenic stress conditions. © 1994 Kluwer Academic Publishers.
Scientific Publication
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