חיפוש מתקדם
Methods in Molecular Biology
Gaba, V., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Lapidot, M., Department of Vegetable Research, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Gal-On, A., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Particle bombardment is an efficient method for virus inoculation of intact plants. This technique enables inoculation with full-length infectious clone cDNA, PCR products, virus from sap or virus preparation, and in vitro viral transcripts. The inoculation of some phloem-limited RNA and circular DNA viruses is also possible. The technique of bombardment without the use of vacuum permits the inoculation of soft-leaved plants that do not usually survive bombardment inoculation, the investigation of viral recombination in planta, promoter analysis, monitoring virus movement using an infectious clone bearing a reporter gene and the inoculation of large numbers of plants. The inoculation of whitefly-borne circular DNA begomoviruses is now possible due to direct genome amplification by Rolling Circle Amplification (RCA), followed by bombardment using a device that does not require a vacuum for operation. Here we describe the inoculation of intact plants with (a) RNA virus infective clones and (b) begomoviruses after direct genome amplification by RCA, using a handheld bombardment device. © 2013 Springer Science+Business Media, LLC.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
HandGun-mediated inoculation of plants with viral pathogens for mechanistic studies
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Gaba, V., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Lapidot, M., Department of Vegetable Research, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Gal-On, A., Department of Plant Pathology and Weed Science, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
HandGun-mediated inoculation of plants with viral pathogens for mechanistic studies
Particle bombardment is an efficient method for virus inoculation of intact plants. This technique enables inoculation with full-length infectious clone cDNA, PCR products, virus from sap or virus preparation, and in vitro viral transcripts. The inoculation of some phloem-limited RNA and circular DNA viruses is also possible. The technique of bombardment without the use of vacuum permits the inoculation of soft-leaved plants that do not usually survive bombardment inoculation, the investigation of viral recombination in planta, promoter analysis, monitoring virus movement using an infectious clone bearing a reporter gene and the inoculation of large numbers of plants. The inoculation of whitefly-borne circular DNA begomoviruses is now possible due to direct genome amplification by Rolling Circle Amplification (RCA), followed by bombardment using a device that does not require a vacuum for operation. Here we describe the inoculation of intact plants with (a) RNA virus infective clones and (b) begomoviruses after direct genome amplification by RCA, using a handheld bombardment device. © 2013 Springer Science+Business Media, LLC.
Scientific Publication
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