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פותח על ידי קלירמאש פתרונות בע"מ -
β-Lactoglobulin/human serum albumin fusion genes do not respond accurately to signals from the extracellular matrix in mammary epithelial cells from transgenic mice
Year:
1996
Source of publication :
Experimental Cell Research
Authors :
אילן, נטע
;
.
ברש, איתמר
;
.
פיירמן, אלכסנדר
;
.
רייכנשטיין, משה
;
.
שני, משה
;
.
Volume :
228
Co-Authors:
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Raikhinstein, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Faerman, A., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
146
To page:
159
(
Total pages:
14
)
Abstract:
Mammary epithelial cell cultures from transgenic mice carrying the human serum albumin (HSA) gene or minigenes behind the regulatory sequences of the ovine β-lactoglobulin gene were analyzed. Previously, we demonstrated that non-HSA-secreting transgenic strains retain the potential to express the HSA transgene in vitro and that mammary epithelial cell cultures from non-HSA- secreting strains express higher levels of HSA when grown on tissue culture plastic than they do when grown on collagen. In this study we studied the expression of BLG/HSA fusion genes in epithelial cell cultures of additional transgenic strains and additional substrata. Our results show that: (1) The BLG/HSA fusion gene in only one of seven HSA-secreting or nonsecreting transgenic strains tested accurately responded to signals from the EHS matrix; (2) HSA DNA sequences dominantly affected the activity of BLG as well as the whey acidic protein promoters; and (3) HGF/SF induced both milk proteins and HSA gene expression. These results suggest that the response to the extra cellular matrix (ECM) plays a key role in the expression of BLG/HSA fusion genes and that the function of the regulatory elements within the promoter regions of milk protein genes involved in response to the ECM, in developmental and in tissue specificity, very much depend on the downstream gene sequences.
Note:
Related Files :
Animals
DNA
Female
gene expression
Growth Substances
mice
pregnancy
Serum Albumin
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/excr.1996.0310
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19037
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
β-Lactoglobulin/human serum albumin fusion genes do not respond accurately to signals from the extracellular matrix in mammary epithelial cells from transgenic mice
228
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Raikhinstein, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Faerman, A., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
β-Lactoglobulin/human serum albumin fusion genes do not respond accurately to signals from the extracellular matrix in mammary epithelial cells from transgenic mice
Mammary epithelial cell cultures from transgenic mice carrying the human serum albumin (HSA) gene or minigenes behind the regulatory sequences of the ovine β-lactoglobulin gene were analyzed. Previously, we demonstrated that non-HSA-secreting transgenic strains retain the potential to express the HSA transgene in vitro and that mammary epithelial cell cultures from non-HSA- secreting strains express higher levels of HSA when grown on tissue culture plastic than they do when grown on collagen. In this study we studied the expression of BLG/HSA fusion genes in epithelial cell cultures of additional transgenic strains and additional substrata. Our results show that: (1) The BLG/HSA fusion gene in only one of seven HSA-secreting or nonsecreting transgenic strains tested accurately responded to signals from the EHS matrix; (2) HSA DNA sequences dominantly affected the activity of BLG as well as the whey acidic protein promoters; and (3) HGF/SF induced both milk proteins and HSA gene expression. These results suggest that the response to the extra cellular matrix (ECM) plays a key role in the expression of BLG/HSA fusion genes and that the function of the regulatory elements within the promoter regions of milk protein genes involved in response to the ECM, in developmental and in tissue specificity, very much depend on the downstream gene sequences.
Scientific Publication
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