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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of an endopolygalacturonase associated with take-all disease of wheat
Year:
1992
Authors :
הרשנהורן, יוסף
;
.
סולל, צבי
;
.
Volume :
40
Co-Authors:
Dori, S., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hershenhorn, J., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel
Solel, Z., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Barash, I., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
203
To page:
210
(
Total pages:
8
)
Abstract:
A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed. © 1992.
Note:
Related Files :
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תוכן קשור
More details
DOI :
10.1016/0885-5765(92)90061-Y
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19092
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
Characterization of an endopolygalacturonase associated with take-all disease of wheat
40
Dori, S., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hershenhorn, J., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel
Solel, Z., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Barash, I., Department of Botany, Tel-Aviv University, Tel Aviv, 69978, Israel, Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Characterization of an endopolygalacturonase associated with take-all disease of wheat
A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed. © 1992.
Scientific Publication
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