נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola
Year:
2012
Source of publication :
Molecular Plant Pathology
Authors :
בלאוסוב, אדוארד
;
.
דרובי, סמיר
;
.
הרשקוביץ, ורה
;
.
עלי, ראדי
;
.
קסלר (בן-דיין), קלריטה
;
.
רפאל, גינת
;
.
Volume :
13
Co-Authors:



Pasmanik-Chor, M., The Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, 69978, Israel
Liu, J., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, 25430, WV, United States


Wisniewski, M., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, 25430, WV, United States
 

Facilitators :
From page:
338
To page:
349
(
Total pages:
12
)
Abstract:
To gain a better understanding of the molecular changes taking place in citrus fruit tissue following the application of the yeast biocontrol agent Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip. Using a cut-off of P < 0.05 and a 1.5-fold change difference as biologically significant, the data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application relative to wounded controls. Microarray results of selected genes were validated by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The data indicated that yeast application induced the expression of the genes encoding Respiratory burst oxidase (Rbo), mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase (MAPKK), G-proteins, chitinase (CHI), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. Moreover, suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. Interestingly, large amounts of hydrogen peroxide were detected inside yeast cells recovered from wounded fruit tissue, indicating the ability of the yeast to activate reactive oxygen species when it is in contact with plant tissue. This study provides the first global picture of gene expression changes in grapefruit in response to the yeast antagonist M.fructicola. © 2011 BSPP and Blackwell Publishing Ltd.
Note:
Related Files :
Genetics
grapefruits
metabolism
Microbiology
reverse transcription polymerase chain reaction
signal transduction
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1364-3703.2011.00750.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19094
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
You may also be interested in
Scientific Publication
Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola
13



Pasmanik-Chor, M., The Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, 69978, Israel
Liu, J., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, 25430, WV, United States


Wisniewski, M., US Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, 25430, WV, United States
 

Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola
To gain a better understanding of the molecular changes taking place in citrus fruit tissue following the application of the yeast biocontrol agent Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip. Using a cut-off of P < 0.05 and a 1.5-fold change difference as biologically significant, the data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application relative to wounded controls. Microarray results of selected genes were validated by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The data indicated that yeast application induced the expression of the genes encoding Respiratory burst oxidase (Rbo), mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase (MAPKK), G-proteins, chitinase (CHI), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. Moreover, suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. Interestingly, large amounts of hydrogen peroxide were detected inside yeast cells recovered from wounded fruit tissue, indicating the ability of the yeast to activate reactive oxygen species when it is in contact with plant tissue. This study provides the first global picture of gene expression changes in grapefruit in response to the yeast antagonist M.fructicola. © 2011 BSPP and Blackwell Publishing Ltd.
Scientific Publication
You may also be interested in