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פותח על ידי קלירמאש פתרונות בע"מ -
Studies on the effects of nuclear background and tissue specificity on RNA editing of the mitochondrial ATP synthase subunits α, 6 and 9 in fertile and cytoplasmic male-sterile (CMS) wheat
Year:
1997
Source of publication :
Theoretical and Applied Genetics
Authors :
עזרא, דוד
;
.
Volume :
95
Co-Authors:
Kurek, I., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Ezra, D., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Begu, D., Laboratoire REGER, IBGC-CNRS, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
Erel, N., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Litvak, S., Laboratoire REGER, IBGC-CNRS, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
Breiman, A., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
1305
To page:
1311
(
Total pages:
7
)
Abstract:
RNA editing occurs in transcripts encoded by the mitochondrial genome in all major groups of land plants and is characterised by C-to-U editing. When editing occurs, the amino acid is usually altered because the nucleotide modification is generally in the first or second position and the amino acid change improves the conservation of the predicted protein as compared with other organisms. In order to assess the effect of the nuclear background and tissue specificity of the RNA editing, we have analysed the RNA editing of the transcripts encoding for the mitochondrial ATPsynthase subunits atpα atp6, and atp9 in euplasmic and alloplasmic cytoplasmic male-sterile (CMS) wheat lines. The editing of atpα was detected in 6 codons. Although partially edited transcripts were found in all the cytoplasms, a larger number of partially edited clones were present in the CMS cytoplasm. The RNA editing of atp6 occurred in 12 codons, and whereas in the euplasmic Triticum timopheevi all the clones were fully edited, in the CMS line 17% of the clones were only partially edited, suggesting an effect of the nuclear background on RNA editing efficiency. In atp9 transcripts, 8 codons were modified by RNA editing. When the RNA editing of the atp9 transcripts extracted from various tissues was compared, only fully edited clones were detected in embryos, roots, shoots, and anthers in the euplasmic wheat lines, whereas in the transcripts obtained from the CMS line, 19% of the clones were partially edited. This study reveals that RNA editing efficiency can be affected by the tissue and nuclear background. The mechanism by which incomplete and complete edited transcripts accumulate may indicate a link between the transcription kinetics and RNA editing which can be affected by nuclear background and tissue specificity.
Note:
Related Files :
ATP synthase
CMS
Editing
Triticum timopheevi
wheat
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s001220050697
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19110
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
Scientific Publication
Studies on the effects of nuclear background and tissue specificity on RNA editing of the mitochondrial ATP synthase subunits α, 6 and 9 in fertile and cytoplasmic male-sterile (CMS) wheat
95
Kurek, I., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Ezra, D., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Begu, D., Laboratoire REGER, IBGC-CNRS, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
Erel, N., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Litvak, S., Laboratoire REGER, IBGC-CNRS, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
Breiman, A., Department of Botany, George S. Wise Fac. of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Studies on the effects of nuclear background and tissue specificity on RNA editing of the mitochondrial ATP synthase subunits α, 6 and 9 in fertile and cytoplasmic male-sterile (CMS) wheat
RNA editing occurs in transcripts encoded by the mitochondrial genome in all major groups of land plants and is characterised by C-to-U editing. When editing occurs, the amino acid is usually altered because the nucleotide modification is generally in the first or second position and the amino acid change improves the conservation of the predicted protein as compared with other organisms. In order to assess the effect of the nuclear background and tissue specificity of the RNA editing, we have analysed the RNA editing of the transcripts encoding for the mitochondrial ATPsynthase subunits atpα atp6, and atp9 in euplasmic and alloplasmic cytoplasmic male-sterile (CMS) wheat lines. The editing of atpα was detected in 6 codons. Although partially edited transcripts were found in all the cytoplasms, a larger number of partially edited clones were present in the CMS cytoplasm. The RNA editing of atp6 occurred in 12 codons, and whereas in the euplasmic Triticum timopheevi all the clones were fully edited, in the CMS line 17% of the clones were only partially edited, suggesting an effect of the nuclear background on RNA editing efficiency. In atp9 transcripts, 8 codons were modified by RNA editing. When the RNA editing of the atp9 transcripts extracted from various tissues was compared, only fully edited clones were detected in embryos, roots, shoots, and anthers in the euplasmic wheat lines, whereas in the transcripts obtained from the CMS line, 19% of the clones were partially edited. This study reveals that RNA editing efficiency can be affected by the tissue and nuclear background. The mechanism by which incomplete and complete edited transcripts accumulate may indicate a link between the transcription kinetics and RNA editing which can be affected by nuclear background and tissue specificity.
Scientific Publication
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