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פותח על ידי קלירמאש פתרונות בע"מ -
Cell suspensions for kinetic studies of inhibitor action - The rapid initial action of dikegulac on membrane properties in situ
Year:
1979
Source of publication :
Planta
Authors :
זילכה, שמואל
;
.
Volume :
145
Co-Authors:
Zilkah, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
273
To page:
278
(
Total pages:
6
)
Abstract:
Because of uniformity and small distances for transport, cell suspensions offer a system for rapid measurements of initial reactions of phytotoxic compounds. We had previously shown that a growth regulator, dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-L-gulonate) inhibits amino acid incorporation into proteins. Using Solanum nigrum suspension cultures, it was found that dikegulac rapidly inhibits amino acid uptake into cells, before inhibiting incorporation, with time points starting at a few minutes, and kinetics that can be extrapolated back to time zero. With more rapid kinetics this compound induces leakage of a preloaded dye. The rate of leakage was less with stationary cells in suspension, reiterating that they are more resistant to the effects of this compound. It was thus concluded that at the concentrations used, the first effect of dikegulac (or one very close to the first effect) is on the cell membrane. © 1979 Springer-Verlag.
Note:
Related Files :
Amino acid uptake
Cell cultures
Cell membranes
Cell suspensions
dikegulac
Leakage
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00454452
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19117
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
Scientific Publication
Cell suspensions for kinetic studies of inhibitor action - The rapid initial action of dikegulac on membrane properties in situ
145
Zilkah, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Cell suspensions for kinetic studies of inhibitor action - The rapid initial action of dikegulac on membrane properties in situ
Because of uniformity and small distances for transport, cell suspensions offer a system for rapid measurements of initial reactions of phytotoxic compounds. We had previously shown that a growth regulator, dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-L-gulonate) inhibits amino acid incorporation into proteins. Using Solanum nigrum suspension cultures, it was found that dikegulac rapidly inhibits amino acid uptake into cells, before inhibiting incorporation, with time points starting at a few minutes, and kinetics that can be extrapolated back to time zero. With more rapid kinetics this compound induces leakage of a preloaded dye. The rate of leakage was less with stationary cells in suspension, reiterating that they are more resistant to the effects of this compound. It was thus concluded that at the concentrations used, the first effect of dikegulac (or one very close to the first effect) is on the cell membrane. © 1979 Springer-Verlag.
Scientific Publication
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