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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Adventitious shoot formation from carnation stem segments: A comparison of different culture procedures
Year:
1996
Source of publication :
Scientia Horticulturae
Authors :
ותד, עבד
;
.
ניסים-לוי, עדה
;
.
Volume :
65
Co-Authors:
Watad, A.A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Ahroni, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zuker, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Shejtman, H., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Nissim, A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Vainstein, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
313
To page:
320
(
Total pages:
8
)
Abstract:
Adventitious shoot regeneration from stem explants of carnation (Dianthus caryophyllus L., cultivar 'White Sim') was studied using three different culture procedures: agar-gelled medium, liquid shaken medium, and an interfacial membrane raft floating on liquid medium. In all culture procedures, explants derived from the first upper internode exhibited higher adventitious shoot formation than those from the second internode. On agar- gelled medium, the number of regenerated shoots per explant from the first internode increased with increasing thidiazuron (TDZ) concentration (up to 4 mg 1-1) in the presence of 1 mg 1-1 naphthaleneacetic acid. In the liquid shaken culture, the number of regenerated shoots peaked at 1 mg TDZ 1-1. Maximum shoot regeneration on an interfacial membrane raft was obtained in the presence of 0.25 mg TDZ 1-1. A comparison of the overall efficiencies of shoot regeneration for each culture procedure showed that maximum shoot regeneration on the raft was twice and three times that obtained with agar- gelled and liquid shaken media, respectively.
Note:
Related Files :
Agar gelled medium
Interfacial membrane raft
Liquid medium
Shoot regeneration
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0304-4238(96)00874-6
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19140
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
Scientific Publication
Adventitious shoot formation from carnation stem segments: A comparison of different culture procedures
65
Watad, A.A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Ahroni, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zuker, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Shejtman, H., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Nissim, A., Dept. of Ornamental Horticulture, Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Vainstein, A., Kennedy-Leigh Ctr. for Hort. R., Hebrew University of Jerusalem, Rehovot 76100, Israel
Adventitious shoot formation from carnation stem segments: A comparison of different culture procedures
Adventitious shoot regeneration from stem explants of carnation (Dianthus caryophyllus L., cultivar 'White Sim') was studied using three different culture procedures: agar-gelled medium, liquid shaken medium, and an interfacial membrane raft floating on liquid medium. In all culture procedures, explants derived from the first upper internode exhibited higher adventitious shoot formation than those from the second internode. On agar- gelled medium, the number of regenerated shoots per explant from the first internode increased with increasing thidiazuron (TDZ) concentration (up to 4 mg 1-1) in the presence of 1 mg 1-1 naphthaleneacetic acid. In the liquid shaken culture, the number of regenerated shoots peaked at 1 mg TDZ 1-1. Maximum shoot regeneration on an interfacial membrane raft was obtained in the presence of 0.25 mg TDZ 1-1. A comparison of the overall efficiencies of shoot regeneration for each culture procedure showed that maximum shoot regeneration on the raft was twice and three times that obtained with agar- gelled and liquid shaken media, respectively.
Scientific Publication
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