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פותח על ידי קלירמאש פתרונות בע"מ -
Analysis of the Ac promoter: Structure and regulation
Year:
1998
Source of publication :
Molecular and General Genetics
Authors :
פרידלנדר, מרסלו
;
.
Volume :
258
Co-Authors:
Fridlender, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, AgBiotech. Center, Rutgers University, New Brunswick, NJ 08903, United States
Sitrit, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Shaul, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Gileadi, O., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Levy, A.A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
306
To page:
314
(
Total pages:
9
)
Abstract:
The Ac-encoded transposase, a factor that is essential for the mobility of the Ac element, is expressed under the control of a promoter that lacks a conventional TATA box. The regulation of this promoter is poorly understood. We have analyzed Ac promoter structure and activity, both in vitro and in vivo, using transgenic tobacco plants and cell suspensions. A deletion analysis of the Ac 5' region showed that the minimal promoter is located within 70 bp of the major transcription initiation site (at position 334). The minimal promoter includes the sequence TAAGAAATA at position 294-303, i.e., about 30 nucleotides upstream from the transcription start site. This sequence binds specifically to the TATA-binding protein (TBP), suggesting that it is functional as a TATA box. The regulation of the Ac promoter was studied throughout plant development. Levels of Ac mRNA were low in all tissues studied, with higher expression being observed in dividing cells. In order to test whether Ac promoter is regulated during the cell cycle, a tobacco cell suspension transformed with Ac, was grown synchronously. No differences were found in Ac mRNA levels between cells in S, G2, M, or G1 phases; however, expression was lower in the stationary phase. We conclude that Ac promoter is not cell-cycle regulated but is expressed at a higher level in dividing cells. The possible relationship between promoter features and the regulation of Ac element transposition is discussed.
Note:
Related Files :
cell cycle G1 phase
cell cycle s phase
gene structure
genetic analysis
Nicotiana obtusifolia
Plants, Toxic
Sequence Deletion
transgenic plant
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s004380050735
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19145
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:26
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Scientific Publication
Analysis of the Ac promoter: Structure and regulation
258
Fridlender, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, AgBiotech. Center, Rutgers University, New Brunswick, NJ 08903, United States
Sitrit, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Shaul, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Gileadi, O., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Levy, A.A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Analysis of the Ac promoter: Structure and regulation
The Ac-encoded transposase, a factor that is essential for the mobility of the Ac element, is expressed under the control of a promoter that lacks a conventional TATA box. The regulation of this promoter is poorly understood. We have analyzed Ac promoter structure and activity, both in vitro and in vivo, using transgenic tobacco plants and cell suspensions. A deletion analysis of the Ac 5' region showed that the minimal promoter is located within 70 bp of the major transcription initiation site (at position 334). The minimal promoter includes the sequence TAAGAAATA at position 294-303, i.e., about 30 nucleotides upstream from the transcription start site. This sequence binds specifically to the TATA-binding protein (TBP), suggesting that it is functional as a TATA box. The regulation of the Ac promoter was studied throughout plant development. Levels of Ac mRNA were low in all tissues studied, with higher expression being observed in dividing cells. In order to test whether Ac promoter is regulated during the cell cycle, a tobacco cell suspension transformed with Ac, was grown synchronously. No differences were found in Ac mRNA levels between cells in S, G2, M, or G1 phases; however, expression was lower in the stationary phase. We conclude that Ac promoter is not cell-cycle regulated but is expressed at a higher level in dividing cells. The possible relationship between promoter features and the regulation of Ac element transposition is discussed.
Scientific Publication
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