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פותח על ידי קלירמאש פתרונות בע"מ -
Isolation and Spectroscopic Characterization of a Plantlike Photosystem II Reaction Center from the Cyanobacterium Synechocystis sp. 6803
Year:
1995
Source of publication :
Biochemistry (source )
Authors :
אורן-שמיר, מיכל
;
.
Volume :
34
Co-Authors:
Michal, O.-S., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Sai, P.S.M., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Scherz, A., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Edelman, M., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Facilitators :
From page:
5523
To page:
5526
(
Total pages:
4
)
Abstract:
A chlorophyll-protein complex has been isolated from the cyanobacterium Synechocystis sp. PCC 6803 that closely resembles higher plant photosystem II reaction centers in spectral properties. The Synechocystis complex has a pigment content of 5-7 chlorophyll a molecules: 1 Cyt 6559:2 pheophytins; an optical absorption redmost transition at ~675 nm; and a nonconservative circular dichroism red signal, with extrema at 682 (+) and 652 (-) nm. Upon illumination, the Synechocystis Dl/D2/Cyt 6559 complex accumulates reduced pheophytin. LDS-PAGE and/or immunoblotting showed the DI, D2, and Cyt 6559 proteins, aggregated and degraded forms of DI and possibly D2, and traces of ATP synthase and the CP47 photosystem II chlorophyll protein. The availability of such a Synechocystis preparation opens the way for employing site-directed mutagenesis in studying primary reactions of oxygenic photosynthesis. © 1995, American Chemical Society. All rights reserved.
Note:
Related Files :
chlorophyll
circular dichroism
Electrophoresis, Polyacrylamide Gel
pheophytin
photosynthesis
spectroscopy
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/bi00016a025
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19191
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:27
You may also be interested in
Scientific Publication
Isolation and Spectroscopic Characterization of a Plantlike Photosystem II Reaction Center from the Cyanobacterium Synechocystis sp. 6803
34
Michal, O.-S., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Sai, P.S.M., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Scherz, A., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Edelman, M., Departments of Biochemistry and Plant Genetics, Weizmann Institute of Science, Rehovot 76100 76100, Israel
Isolation and Spectroscopic Characterization of a Plantlike Photosystem II Reaction Center from the Cyanobacterium Synechocystis sp. 6803
A chlorophyll-protein complex has been isolated from the cyanobacterium Synechocystis sp. PCC 6803 that closely resembles higher plant photosystem II reaction centers in spectral properties. The Synechocystis complex has a pigment content of 5-7 chlorophyll a molecules: 1 Cyt 6559:2 pheophytins; an optical absorption redmost transition at ~675 nm; and a nonconservative circular dichroism red signal, with extrema at 682 (+) and 652 (-) nm. Upon illumination, the Synechocystis Dl/D2/Cyt 6559 complex accumulates reduced pheophytin. LDS-PAGE and/or immunoblotting showed the DI, D2, and Cyt 6559 proteins, aggregated and degraded forms of DI and possibly D2, and traces of ATP synthase and the CP47 photosystem II chlorophyll protein. The availability of such a Synechocystis preparation opens the way for employing site-directed mutagenesis in studying primary reactions of oxygenic photosynthesis. © 1995, American Chemical Society. All rights reserved.
Scientific Publication
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