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פותח על ידי קלירמאש פתרונות בע"מ -
Similarity of the ruminal bacteria across individual lactating cows
Year:
2012
Source of publication :
Anaerobe
Authors :
ז'מי, אלי
;
.
Volume :
18
Co-Authors:
Jami, E., Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel, Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Science, Tel Aviv University, Ramat-Aviv 69978, Israel
Mizrahi, I., Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
338
To page:
343
(
Total pages:
6
)
Abstract:
Dairy cattle hold enormous significance for man as a source of milk and meat. Their remarkable ability to convert indigestible plant mass into these digestible food products resides in the rumen - an anaerobic chambered compartment - in the bovine digestive system. The rumen houses a complex microbiota which is responsible for the degradation of plant material, consequently enabling the conversion of plant fibers into milk and meat and determining their quality and quantity. Hence, an understanding of this complex ecosystem has major economic implications. One important question that is yet to be addressed is the degree of conservation of rumen microbial composition across individual animals. Here we quantified the degree of similarity between rumen bacterial populations of 16 individual cows. We used real-time PCR to determine the variance of specific ruminal bacterial species with different metabolic functions, revealing that while some bacterial strains vary greatly across animals, others show only very low variability. This variance could not be linked to the metabolic traits of these bacteria. We examined the degree of similarity in the dominant bacterial populations across all animals using automated ribosomal intergenic spacer analysis (ARISA), and identified a bacterial community consisting of 32% operational taxonomic units (OTUs) shared by at least 90% of the animals and 19% OTUs shared by 100% of the animals. Looking only at the presence or absence of each OTU gave an average similarity of 75% between each cow pair. When abundance of each OTU was added to the analysis, this similarity decreased to an average of less than 60%. Thus, as suggested in similar recent studies of the human gut, a bovine rumen core microbiome does exist, but taxa abundance may vary greatly across animals. © 2012 Elsevier Ltd.
Note:
Related Files :
Animals
cattle
Female
lactation
microbial ecology
RT-PCR (Real-Time Polymerase Chain Reaction)
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.anaerobe.2012.04.003
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19201
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:27
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Scientific Publication
Similarity of the ruminal bacteria across individual lactating cows
18
Jami, E., Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel, Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Science, Tel Aviv University, Ramat-Aviv 69978, Israel
Mizrahi, I., Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Similarity of the ruminal bacteria across individual lactating cows
Dairy cattle hold enormous significance for man as a source of milk and meat. Their remarkable ability to convert indigestible plant mass into these digestible food products resides in the rumen - an anaerobic chambered compartment - in the bovine digestive system. The rumen houses a complex microbiota which is responsible for the degradation of plant material, consequently enabling the conversion of plant fibers into milk and meat and determining their quality and quantity. Hence, an understanding of this complex ecosystem has major economic implications. One important question that is yet to be addressed is the degree of conservation of rumen microbial composition across individual animals. Here we quantified the degree of similarity between rumen bacterial populations of 16 individual cows. We used real-time PCR to determine the variance of specific ruminal bacterial species with different metabolic functions, revealing that while some bacterial strains vary greatly across animals, others show only very low variability. This variance could not be linked to the metabolic traits of these bacteria. We examined the degree of similarity in the dominant bacterial populations across all animals using automated ribosomal intergenic spacer analysis (ARISA), and identified a bacterial community consisting of 32% operational taxonomic units (OTUs) shared by at least 90% of the animals and 19% OTUs shared by 100% of the animals. Looking only at the presence or absence of each OTU gave an average similarity of 75% between each cow pair. When abundance of each OTU was added to the analysis, this similarity decreased to an average of less than 60%. Thus, as suggested in similar recent studies of the human gut, a bovine rumen core microbiome does exist, but taxa abundance may vary greatly across animals. © 2012 Elsevier Ltd.
Scientific Publication
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