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פותח על ידי קלירמאש פתרונות בע"מ -
Amplified degradation of photosystem II D1 and D2 proteins under a mixture of photosynthetically active radiation and UVB radiation: Dependence on redox status of photosystem II
Year:
1999
Source of publication :
Photochemistry and Photobiology
Authors :
גאבה, ויקטור
;
.
Volume :
69
Co-Authors:
Babu, T.S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Biology, University of Waterloo, Waterloo, Ont., Canada, Department of Biology, University of Waterloo, Waterloo, Ont. N2L 3G1, Canada
Jansen, M.A.K., Department of Plant Physiology, Agricultural University, Wageningen, Netherlands, Nitrogen Fixation Laboratory, John Innes Centre, Colney Lane, Colney, Norwich NR4 7UH, United Kingdom
Greenberg, B.M., Department of Biology, University of Waterloo, Waterloo, Ont., Canada
Gaba, V., Department of Virology, ARO Volcani Center, Bet Dagan, Israel
Malkin, S., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Mattoo, A.K., Vegetable Laboratory, Agricultural Research Center, USDA/ARS, Beltsville, MD, United States
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
553
To page:
559
(
Total pages:
7
)
Abstract:
Plants exposed to a mixture of photosynthetically active radiation (PAR) and UVB radiation exhibit a marked boost in degradation of the D1 and D2 photosystem II (PS II) reaction center proteins beyond that predicted by the sum of rates in PAR and UVB alone (amplified degradation). Becausee degradation driven by visible or UVB radiation alone is uncoupled from PS II redox status, it was therefore assumed that the mixed-light-amplified component of degradation would behave similarly. Surprisingly, amplified degradation proved to be coupled tightly to the redox status of PS II. We show that inactivation of the PS II water oxidation by heat shock or oxidation of the plastosemiquinone (QA ) by silicomolybdate nullifies only the amplified component of degradation but not the basic rates of degradation under PAR or UVB alone. The data are interpreted to indicate that formation of plastosemiquinone or an active water-oxidizing Mn4 cluster, is the UVB chromophore involved in amplified degradation of the D1 and D2 proteins. Furthermore, accumulation of QA- by 3-(3,4-dichlorophenyl)-1,1-dimethylurea or 2-bromo-3-methyl-6-isopropyl-4-nitrophenol stimulated the mixed-light-amplified degradation component. Thus, amplified degradation of the D1 and D2 proteins in mixed radiance of PAR plus UVB (which simulates naturally occurring radiance) proceeds by a mechanism clearly distinct from that involved in degradation under PAR or UVB alone.
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DOI :
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Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19205
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:27
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Scientific Publication
Amplified degradation of photosystem II D1 and D2 proteins under a mixture of photosynthetically active radiation and UVB radiation: Dependence on redox status of photosystem II
69
Babu, T.S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Biology, University of Waterloo, Waterloo, Ont., Canada, Department of Biology, University of Waterloo, Waterloo, Ont. N2L 3G1, Canada
Jansen, M.A.K., Department of Plant Physiology, Agricultural University, Wageningen, Netherlands, Nitrogen Fixation Laboratory, John Innes Centre, Colney Lane, Colney, Norwich NR4 7UH, United Kingdom
Greenberg, B.M., Department of Biology, University of Waterloo, Waterloo, Ont., Canada
Gaba, V., Department of Virology, ARO Volcani Center, Bet Dagan, Israel
Malkin, S., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Mattoo, A.K., Vegetable Laboratory, Agricultural Research Center, USDA/ARS, Beltsville, MD, United States
Edelman, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Amplified degradation of photosystem II D1 and D2 proteins under a mixture of photosynthetically active radiation and UVB radiation: Dependence on redox status of photosystem II
Plants exposed to a mixture of photosynthetically active radiation (PAR) and UVB radiation exhibit a marked boost in degradation of the D1 and D2 photosystem II (PS II) reaction center proteins beyond that predicted by the sum of rates in PAR and UVB alone (amplified degradation). Becausee degradation driven by visible or UVB radiation alone is uncoupled from PS II redox status, it was therefore assumed that the mixed-light-amplified component of degradation would behave similarly. Surprisingly, amplified degradation proved to be coupled tightly to the redox status of PS II. We show that inactivation of the PS II water oxidation by heat shock or oxidation of the plastosemiquinone (QA ) by silicomolybdate nullifies only the amplified component of degradation but not the basic rates of degradation under PAR or UVB alone. The data are interpreted to indicate that formation of plastosemiquinone or an active water-oxidizing Mn4 cluster, is the UVB chromophore involved in amplified degradation of the D1 and D2 proteins. Furthermore, accumulation of QA- by 3-(3,4-dichlorophenyl)-1,1-dimethylurea or 2-bromo-3-methyl-6-isopropyl-4-nitrophenol stimulated the mixed-light-amplified degradation component. Thus, amplified degradation of the D1 and D2 proteins in mixed radiance of PAR plus UVB (which simulates naturally occurring radiance) proceeds by a mechanism clearly distinct from that involved in degradation under PAR or UVB alone.
Scientific Publication
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