נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The tomato hexokinase LeHXK1 cloning, mapping, expression pattern and phylogenetic relationships
Year:
2002
Source of publication :
Plant Science
Authors :
גרנות, דוד
;
.
דאי, ניר
;
.
חנאל, רנה
;
.
לוין, אילן
;
.
פטרייקוב, מרינה
;
.
קנדל-כפיר, מיכל
;
.
שפר, ארתור
;
.
Volume :
163
Co-Authors:
Dai, N., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kandel-Kfir, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Petreikov, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hanael, R., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Levin, I., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ricard, B., Plant Physiology Laboratory, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France
Rothan, C., Plant Physiology Laboratory, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France
Schaffer, A.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
581
To page:
590
(
Total pages:
10
)
Abstract:
A tomato (Lycopersicon esculentum L. Mill.) hexokinase, LeHXK1, was cloned from a tomato fruit cDNA library. Functional complementation of triple-mutant yeast cells (hxk1 hxk2 glk1) that lack the ability to phosphorylate glucose and fructose confirmed the identity of LeHXK1 as a hexokinase. LeHXK1 was mapped to tomato chromosome 3-distinct from LeHXK2 which mapped to chromosome 6. Interestingly, expression of LeHXK1 was very low in leaves and very high in young tomato fruits. Two hexokinase isozymes, HXK1 and HXK2, were chromatographically separated from young fruit extracts, Antisense StHXK1 the homologue of, LeHXK1, knocked out the HXK1 isozyme, identifying HXK1 as the gene product of LeHXK1. Phylogenetic analysis of eukaryotic and prokaryotic hexose phosphorylating enzymes indicates that tomato hexokinase genes, LeHXK1 and LeHXK2, and the known eukaryotic hexokinases and 'glucokinases' belong to the same hexokinase family whose members are unrelated to the glucose-specific glucokinases of bacteria. Hence, the data suggest that thus far no true eukaryotic glucokinases have been identified, neither from plants, nor from fungi or mammalian cells. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Note:
Related Files :
arabidopsis
bacteria
cloning
DNA
enzymes
fungi
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0168-9452(02)00166-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19221
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:27
Scientific Publication
The tomato hexokinase LeHXK1 cloning, mapping, expression pattern and phylogenetic relationships
163
Dai, N., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kandel-Kfir, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Petreikov, M., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Hanael, R., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Levin, I., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ricard, B., Plant Physiology Laboratory, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France
Rothan, C., Plant Physiology Laboratory, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France
Schaffer, A.A., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
The tomato hexokinase LeHXK1 cloning, mapping, expression pattern and phylogenetic relationships
A tomato (Lycopersicon esculentum L. Mill.) hexokinase, LeHXK1, was cloned from a tomato fruit cDNA library. Functional complementation of triple-mutant yeast cells (hxk1 hxk2 glk1) that lack the ability to phosphorylate glucose and fructose confirmed the identity of LeHXK1 as a hexokinase. LeHXK1 was mapped to tomato chromosome 3-distinct from LeHXK2 which mapped to chromosome 6. Interestingly, expression of LeHXK1 was very low in leaves and very high in young tomato fruits. Two hexokinase isozymes, HXK1 and HXK2, were chromatographically separated from young fruit extracts, Antisense StHXK1 the homologue of, LeHXK1, knocked out the HXK1 isozyme, identifying HXK1 as the gene product of LeHXK1. Phylogenetic analysis of eukaryotic and prokaryotic hexose phosphorylating enzymes indicates that tomato hexokinase genes, LeHXK1 and LeHXK2, and the known eukaryotic hexokinases and 'glucokinases' belong to the same hexokinase family whose members are unrelated to the glucose-specific glucokinases of bacteria. Hence, the data suggest that thus far no true eukaryotic glucokinases have been identified, neither from plants, nor from fungi or mammalian cells. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Scientific Publication
You may also be interested in