Co-Authors:
Nadel, B., Department of Horticulture, Faculty of Agriculture, Hebrew University, Rehovot and Department of Fruit Breeding and Genetics, A.R.O., The Volcani Center, Bet Dagan, Israel
Spiegel-Roy, P., Department of Horticulture, Faculty of Agriculture, Hebrew University, Rehovot and Department of Fruit Breeding and Genetics, A.R.O., The Volcani Center, Bet Dagan, Israel
Abstract:
Embryogenic cell cultures of Villafranca lemon capable of growth were selected in the presence of toxin produced by the fungus Phoma tracheiphila, the causal agent of Mal secco. Mal secco is a serious tracheomycotic disease of lemon (Citrus limon Burm. f.) and citron (C. medica L.). Rigorous clonal selection with the variant line was conducted for six consecutive subcultures. Viability of cell lines was monitored by the use of fluorescein diacetate. The stability of the resistance was examined after growth on non-selective medium for three subcultures. The variant line Var. 1.117 showed stable resistance. Cells of the resistant variant line maintained their enbryogenic capacity. Callus induced from the resulting somatic embryos also displayed resistance to the toxin. © 1987.
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