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Potato Research
Nachmias, A., Department of Plant Pathology, Agricultural Research Organization, Gilat Experiment Station, Negev, Israel
Buchner, V., Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel
Krikun, J., Department of Plant Pathology, Agricultural Research Organization, Gilat Experiment Station, Negev, Israel
An extracellular protein-lipopolysaccaride antigen (PLP) was purified from culture fluids of Verticillium dahliae. Antiserum produced in rabbits to the PLP detected the antigen in homogenates of tubers, stems and leaves of V. dahliae-infected potato plants but not in extracts of healthy potato tissue or extracts of potato plants infected by other fungal pathogens. The antigen was not detectable in extracts of potato isolates of V. tricorpus, V. nigrescens and V. nubilum or various other fungi. The antigen was shown to be different from cross-reactive antigens detected by antisera to mycelial antigens. When used as a tool for specific diagnosis of V. dahliae infection in potato, antiserum to PLP was more reliable than that prepared against fungal body antigens. © 1982 Kluwer Academic Publishers.
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Differential diagnosis of Verticillium dahliae in potato with antisera to partially purified pathogen-produced extracellular antigens
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Nachmias, A., Department of Plant Pathology, Agricultural Research Organization, Gilat Experiment Station, Negev, Israel
Buchner, V., Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel
Krikun, J., Department of Plant Pathology, Agricultural Research Organization, Gilat Experiment Station, Negev, Israel
Differential diagnosis of Verticillium dahliae in potato with antisera to partially purified pathogen-produced extracellular antigens
An extracellular protein-lipopolysaccaride antigen (PLP) was purified from culture fluids of Verticillium dahliae. Antiserum produced in rabbits to the PLP detected the antigen in homogenates of tubers, stems and leaves of V. dahliae-infected potato plants but not in extracts of healthy potato tissue or extracts of potato plants infected by other fungal pathogens. The antigen was not detectable in extracts of potato isolates of V. tricorpus, V. nigrescens and V. nubilum or various other fungi. The antigen was shown to be different from cross-reactive antigens detected by antisera to mycelial antigens. When used as a tool for specific diagnosis of V. dahliae infection in potato, antiserum to PLP was more reliable than that prepared against fungal body antigens. © 1982 Kluwer Academic Publishers.
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