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פותח על ידי קלירמאש פתרונות בע"מ -
Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus
Year:
1992
Source of publication :
Virology
Authors :
גפני, רון
;
.
לפידות, משה
;
.
Volume :
187
Co-Authors:
Gafny, R., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Lapidot, M., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Berna, A., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Holt, C.A., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Deom, C.M., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Beachy, R.N., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Facilitators :
From page:
499
To page:
507
(
Total pages:
9
)
Abstract:
A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutations on local and systemic movements of the infection was evaluated. Deletion of 9 or 33 amino acids from the carboxy terminus of the movement protein did not effect cell-to-cell movement as reflected by local lesion formation on Nicotiana tabacum cv. Xanthi NN plants. Deletion of 55 amino acids resulted in impaired MP that supported the formation of local lesions of 1 mm in diameter compared to lesions of 3-5 mm caused by the wild-type MP. Deletion of 74 amino acids (or more) from the carboxy terminus resulted in a protein that could not support virus movement. Modified viruses that contained repeated sequences in the 3′ region of the MP gene lost the repeated sequences during replication and reverted to the wild type. This was evidenced by the size of the MP produced and by sequence analysis of reverse-transcribed PCR-amplified products, following infection by the modified virus. MP deleted for as few as 3 amino acids at the amino terminus could not support virus movement thus indicating that the amino-terminal domain is critical for MP activity. © 1992.
Note:
Related Files :
chromosome deletion
gene amplification
Plants, Toxic
Tobacco mosaic virus
virus gene
virus replication
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0042-6822(92)90452-U
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19365
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
You may also be interested in
Scientific Publication
Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus
187
Gafny, R., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Lapidot, M., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Berna, A., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Holt, C.A., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Deom, C.M., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Beachy, R.N., Department of Biology, Washington University, Campus Box 1137, St. Louis, MO 63130, United States
Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus
A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutations on local and systemic movements of the infection was evaluated. Deletion of 9 or 33 amino acids from the carboxy terminus of the movement protein did not effect cell-to-cell movement as reflected by local lesion formation on Nicotiana tabacum cv. Xanthi NN plants. Deletion of 55 amino acids resulted in impaired MP that supported the formation of local lesions of 1 mm in diameter compared to lesions of 3-5 mm caused by the wild-type MP. Deletion of 74 amino acids (or more) from the carboxy terminus resulted in a protein that could not support virus movement. Modified viruses that contained repeated sequences in the 3′ region of the MP gene lost the repeated sequences during replication and reverted to the wild type. This was evidenced by the size of the MP produced and by sequence analysis of reverse-transcribed PCR-amplified products, following infection by the modified virus. MP deleted for as few as 3 amino acids at the amino terminus could not support virus movement thus indicating that the amino-terminal domain is critical for MP activity. © 1992.
Scientific Publication
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