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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Identification, functional characterization, and evolution of terpene synthases from a basal dicot
Year:
2015
Authors :
אבדאח, מוופק
;
.
בר, עינת
;
.
דוידוביץ'-רקנאטי, רחל
;
.
דורון-פייגנבאום, עדי
;
.
יאהיה, מוסעב
;
.
לוינסון, אפרים
;
.
Volume :
169
Co-Authors:
Yahyaa, M., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Matsuba, Y., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Brandt, W., Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle Saale, Germany
Doron-Faigenboim, A., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Bar, E., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
McClain, A., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Davidovich-Rikanati, R., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Lewinsohn, E., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Pichersky, E., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Ibdah, M., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Facilitators :
From page:
1683
To page:
1697
(
Total pages:
15
)
Abstract:
Bay laurel (Laurus nobilis) is an agriculturally and economically important dioecious tree in the basal dicot family Lauraceae used in food and drugs and in the cosmetics industry. Bay leaves, with their abundant monoterpenes and sesquiterpenes, are used to impart flavor and aroma to food, and have also drawn attention in recent years because of their potential pharmaceutical applications. To identify terpene synthases (TPSs) involved in the production of these volatile terpenes, we performed RNA sequencing to profile the transcriptome of L. nobilis leaves. Bioinformatic analysis led to the identification of eight TPS complementary DNAs. We characterized the enzymes encoded by three of these complementary DNAs: a monoterpene synthase that belongs to the TPS-b clade catalyzes the formation of mostly 1,8-cineole; a sesquiterpene synthase belonging to the TPS-a clade catalyzes the formation of mainly cadinenes; and a diterpene synthase of the TPS-e/f clade catalyzes the formation of geranyllinalool. Comparison of the sequences of these three TPSs indicated that the TPS-a and TPS-b clades of the TPS gene family evolved early in the evolution of the angiosperm lineage, and that geranyllinalool synthase activity is the likely ancestral function in angiosperms of genes belonging to an ancient TPS-e/f subclade that diverged from the kaurene synthase gene lineages before the split of angiosperms and gymnosperms. © 2015 American Society of Plant Biologists. All rights Reserved.
Note:
Related Files :
Base Sequence
Genetics
metabolism
Molecular Evolution
molecular genetics
terpenes
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.15.00930
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19395
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:28
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Scientific Publication
Identification, functional characterization, and evolution of terpene synthases from a basal dicot
169
Yahyaa, M., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Matsuba, Y., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Brandt, W., Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle Saale, Germany
Doron-Faigenboim, A., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Bar, E., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
McClain, A., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Davidovich-Rikanati, R., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Lewinsohn, E., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Pichersky, E., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, United States
Ibdah, M., Newe Yaar Research Center, Agriculture Research Organization, Ramat Yishay, Israel
Identification, functional characterization, and evolution of terpene synthases from a basal dicot
Bay laurel (Laurus nobilis) is an agriculturally and economically important dioecious tree in the basal dicot family Lauraceae used in food and drugs and in the cosmetics industry. Bay leaves, with their abundant monoterpenes and sesquiterpenes, are used to impart flavor and aroma to food, and have also drawn attention in recent years because of their potential pharmaceutical applications. To identify terpene synthases (TPSs) involved in the production of these volatile terpenes, we performed RNA sequencing to profile the transcriptome of L. nobilis leaves. Bioinformatic analysis led to the identification of eight TPS complementary DNAs. We characterized the enzymes encoded by three of these complementary DNAs: a monoterpene synthase that belongs to the TPS-b clade catalyzes the formation of mostly 1,8-cineole; a sesquiterpene synthase belonging to the TPS-a clade catalyzes the formation of mainly cadinenes; and a diterpene synthase of the TPS-e/f clade catalyzes the formation of geranyllinalool. Comparison of the sequences of these three TPSs indicated that the TPS-a and TPS-b clades of the TPS gene family evolved early in the evolution of the angiosperm lineage, and that geranyllinalool synthase activity is the likely ancestral function in angiosperms of genes belonging to an ancient TPS-e/f subclade that diverged from the kaurene synthase gene lineages before the split of angiosperms and gymnosperms. © 2015 American Society of Plant Biologists. All rights Reserved.
Scientific Publication
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