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פותח על ידי קלירמאש פתרונות בע"מ -
Cloning and restriction analysis of ribosomal RNA genes from Mycobacterium smegmatis
Year:
1989
Source of publication :
FEMS Microbiology Letters
Authors :
סלע, שלמה
;
.
Volume :
57
Co-Authors:
Bercovier, H., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kafri, O., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kornitzer, D., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Sela, S., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Facilitators :
From page:
125
To page:
128
(
Total pages:
4
)
Abstract:
A genomic library of Mycobacterium smegmatis DNA was constructed in phage EMBL3. A clone (λ HB85) containing rRNA genes was isolated using as probes, fragments of E. coli rRNA cistron B. This cloned DNA fragment was mapped by restriction analysis and was shown to contain one complete set of rRNA genes (rRNA A). The physical mapping of the second set of rRNA genes of M. smegmatis (rRNA B) was done by restriction analysis of total chromosomal DNA. The two sets of rRNA genes showed highly conserved restriction sites within the respective sets but not in the flanking regions. The two rRNA sets of genes are organised like in the other eubacteria in the order 16S-23S-5S. © 1989.
Note:
Related Files :
bacterial RNA
DNA, Ribosomal
genetic engineering
Genetics
Mycobacterium
Ribosomal RNA genes
RNA
RNA, Ribosomal
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19438
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
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Scientific Publication
Cloning and restriction analysis of ribosomal RNA genes from Mycobacterium smegmatis
57
Bercovier, H., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kafri, O., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kornitzer, D., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Sela, S., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Cloning and restriction analysis of ribosomal RNA genes from Mycobacterium smegmatis
A genomic library of Mycobacterium smegmatis DNA was constructed in phage EMBL3. A clone (λ HB85) containing rRNA genes was isolated using as probes, fragments of E. coli rRNA cistron B. This cloned DNA fragment was mapped by restriction analysis and was shown to contain one complete set of rRNA genes (rRNA A). The physical mapping of the second set of rRNA genes of M. smegmatis (rRNA B) was done by restriction analysis of total chromosomal DNA. The two sets of rRNA genes showed highly conserved restriction sites within the respective sets but not in the flanking regions. The two rRNA sets of genes are organised like in the other eubacteria in the order 16S-23S-5S. © 1989.
Scientific Publication
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