אסיף מאגר המחקר החקלאי

Cloning and restriction analysis of ribosomal RNA genes from Mycobacterium smegmatis

Year:

1989

Source of publication :

Authors :

Volume :

57

Co-Authors:

Bercovier, H., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kafri, O., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Kornitzer, D., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
Sela, S., Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem, Israel

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Abstract:

A genomic library of Mycobacterium smegmatis DNA was constructed in phage EMBL3. A clone (λ HB85) containing rRNA genes was isolated using as probes, fragments of E. coli rRNA cistron B. This cloned DNA fragment was mapped by restriction analysis and was shown to contain one complete set of rRNA genes (rRNA A). The physical mapping of the second set of rRNA genes of M. smegmatis (rRNA B) was done by restriction analysis of total chromosomal DNA. The two sets of rRNA genes showed highly conserved restriction sites within the respective sets but not in the flanking regions. The two rRNA sets of genes are organised like in the other eubacteria in the order 16S-23S-5S. © 1989.

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