חיפוש מתקדם
Transgenic Research
Hamamouch, N., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Westwood, J.H., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Banner, I., Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Cramer, C.L., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Gepstein, S., Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Aly, R., Agricultural Research Organization (ARO), Department of Weed Research, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Parasitic plants present some of the most intractable weed problems for agriculture in much of the world. Species of root parasites such as Orobanche can cause enormous yield losses, yet few control measures are effective and affordable. An ideal solution to this problem is the development of parasite-resistant crops, but this goal has been elusive for most susceptible crops. Here we report a mechanism for resistance to the parasitic angiosperm Orobanche based on expression of sarcotoxin IA in transgenic tobacco. Sarcotoxin IA is a 40-residue peptide with antibiotic activity, originally isolated from the fly, Sarcophaga peregrina. The sarcotoxin IA gene was fused to an Orobanche-inducible promoter, HMG2, which is induced locally in the host root at the point of contact with the parasite, and used to transform tobacco. The resulting transgenic plants accumulated more biomass than non-transformed plants in the presence of parasites. Furthermore, plants expressing sarcotoxin IA showed enhanced resistance to O. aegyptiaca as evidenced by abnormal parasite development and higher parasite mortality after attachment as compared to non-transformed plants. The transgenic plants were similar in appearance to non-transformed plants suggesting that sarcotoxin IA is not detrimental to the host. © Springer 2005.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
A peptide from insects protects transgenic tobacco from a parasitic weed
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Hamamouch, N., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Westwood, J.H., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Banner, I., Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Cramer, C.L., Virginia Tech., Department of Plant Pathology, Physiology, and Weed Science, Blacksburg, VA 24061, United States
Gepstein, S., Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
Aly, R., Agricultural Research Organization (ARO), Department of Weed Research, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
A peptide from insects protects transgenic tobacco from a parasitic weed
Parasitic plants present some of the most intractable weed problems for agriculture in much of the world. Species of root parasites such as Orobanche can cause enormous yield losses, yet few control measures are effective and affordable. An ideal solution to this problem is the development of parasite-resistant crops, but this goal has been elusive for most susceptible crops. Here we report a mechanism for resistance to the parasitic angiosperm Orobanche based on expression of sarcotoxin IA in transgenic tobacco. Sarcotoxin IA is a 40-residue peptide with antibiotic activity, originally isolated from the fly, Sarcophaga peregrina. The sarcotoxin IA gene was fused to an Orobanche-inducible promoter, HMG2, which is induced locally in the host root at the point of contact with the parasite, and used to transform tobacco. The resulting transgenic plants accumulated more biomass than non-transformed plants in the presence of parasites. Furthermore, plants expressing sarcotoxin IA showed enhanced resistance to O. aegyptiaca as evidenced by abnormal parasite development and higher parasite mortality after attachment as compared to non-transformed plants. The transgenic plants were similar in appearance to non-transformed plants suggesting that sarcotoxin IA is not detrimental to the host. © Springer 2005.
Scientific Publication
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