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פותח על ידי קלירמאש פתרונות בע"מ -
Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits
Year:
2007
Source of publication :
Transgenic Research
Authors :
אור, אתי
;
.
סהר, נחמן
;
.
פליישמן, משה
;
.
פרל, אבי
;
.
Volume :
16
Co-Authors:
Hanania, U., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Velcheva, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel, Alberta Research Council, Hwy 16A and 75 Street, PO Bag 4000, Vegreville, Alta. T9C 1T4, Canada
Or, E., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Flaishman, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Sahar, N., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Perl, A., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
515
To page:
525
(
Total pages:
11
)
Abstract:
Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that participate in the seedlessness machinery. Two 'Thompson' lines, a seeded and a seedless, were screened during different flower developmental stages. One of the genes, that was differentially expressed between the seeded and seedless lines, was the chloroplast chaperonin 21 (ch-Cpn21). ch-Cpn21 is a 21-kDa co-chaperonin polypeptide formed by two GroES-like domains fused together in tandem. Silencing of ch-Cpn21 in Nicotiana benthamiana plants resulted in leaf stunting, chlorosis, as well as ovary necrogenesis leading to seed abortion. Moreover, organ-specific silencing of ch-Cpn21 only in Lycopersicum esculentum fruits resulted in the development of seedless tomatoes. These results suggest that ch-Cpn21 may play a role in seed abortion in stenospermocarpic grapes. © 2006 Springer Science+Business Media B.V.
Note:
Related Files :
gene silencing
Genetics
Growth, Development and Aging
metabolism
seeds
Vitis
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s11248-006-9044-0
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19521
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
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Scientific Publication
Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits
16
Hanania, U., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Velcheva, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel, Alberta Research Council, Hwy 16A and 75 Street, PO Bag 4000, Vegreville, Alta. T9C 1T4, Canada
Or, E., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Flaishman, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Sahar, N., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Perl, A., Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan 50250, Israel
Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits
Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that participate in the seedlessness machinery. Two 'Thompson' lines, a seeded and a seedless, were screened during different flower developmental stages. One of the genes, that was differentially expressed between the seeded and seedless lines, was the chloroplast chaperonin 21 (ch-Cpn21). ch-Cpn21 is a 21-kDa co-chaperonin polypeptide formed by two GroES-like domains fused together in tandem. Silencing of ch-Cpn21 in Nicotiana benthamiana plants resulted in leaf stunting, chlorosis, as well as ovary necrogenesis leading to seed abortion. Moreover, organ-specific silencing of ch-Cpn21 only in Lycopersicum esculentum fruits resulted in the development of seedless tomatoes. These results suggest that ch-Cpn21 may play a role in seed abortion in stenospermocarpic grapes. © 2006 Springer Science+Business Media B.V.
Scientific Publication
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