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פותח על ידי קלירמאש פתרונות בע"מ -
A reference map of Cucumis melo based on two recombinant inbred line populations
Year:
2002
Source of publication :
Theoretical and Applied Genetics
Authors :
פורטנוי, ויטלי
;
.
קציר, נורית
;
.
Volume :
104
Co-Authors:
Périn, C., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
Hagen, L.S., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
De Conto, V., SEMINIS Research, Mas de Rouzel, chemin des Canaux, 30900 Nîmes, France
Katzir, N., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Danin-Poleg, Y., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Portnoy, V., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Baudracco-Arnas, S., ASL, Site Agroparc, B.P. 1202, 84911 Avignon Cedex 9, France
Chadoeuf, J., INRA, Unité de Biométrie, Domaine St Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Dogimont, C., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
Pitrat, M., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
Facilitators :
From page:
1017
To page:
1034
(
Total pages:
18
)
Abstract:
A composite genetic melon map was generated based on two recombinant inbred line (RI) populations. By analyzing the segregation of 346 AFLPs, 113 IMAs and phenotypic characters on a RI population of 163 individuals derived from the cross Védrantais x PI 161375, a first map was constructed. About 20% of the molecular markers were skewed, and the residual heterozygosity was estimated at 4.43% which was not significantly different from the theoretical value of 4.2%. The genome distribution of molecular markers among the 12 linkage groups was not different from a random distribution with the exception of linkage group XII which was found significantly less populated. The genome distributions of IMAs and AFLPs were complementary. AFLPs were found mainly in the middle of each linkage group and sometimes clustered, whereas IMAs were found mainly at the end. A total of 318 molecular markers, mainly AFLP and IMA markers, were mapped on 63 RIs of the second population, Védrantais x PI 414723. Comparison of the maps enables one to conclude that AFLPs and IMAs of like molecular size, amplified with the same primer combination, correspond to the same genetic locus. Both maps were joined through 116 common markers comprising 106 comigrating AFLPs/IMAs, plus five SSRs and five phenotypic markers. The integrated melon map contained 668 loci issuing from the segregation of 1,093 molecular markers in the two RI populations. The composite map spanned 1,654 cM on 12 linkage groups which is the haploid number of chromosomes in melon. Thirty two known-function probes, i.e. known-function genes (9) and morphological traits (23), were included in this map. In addition, the composite map was anchored to previously published maps through SSRs, RFLPs and phenotypic characters.
Note:
Related Files :
AFLP
Cucumis melo
Fruits
genetic engineering
Genetic mapping
Genetic maps
IMA
Population statistics
RIL
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-002-0864-x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19535
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:29
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Scientific Publication
A reference map of Cucumis melo based on two recombinant inbred line populations
104
Périn, C., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
Hagen, L.S., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
De Conto, V., SEMINIS Research, Mas de Rouzel, chemin des Canaux, 30900 Nîmes, France
Katzir, N., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Danin-Poleg, Y., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Portnoy, V., ARO, Newe Ya'ar Research Center, Ramat Yishay 30095, Israel
Baudracco-Arnas, S., ASL, Site Agroparc, B.P. 1202, 84911 Avignon Cedex 9, France
Chadoeuf, J., INRA, Unité de Biométrie, Domaine St Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Dogimont, C., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
Pitrat, M., INRA, Stn. Genet./d'A. Fruits/Legumes, BP 94, 84143 Montfavet Cedex, France
A reference map of Cucumis melo based on two recombinant inbred line populations
A composite genetic melon map was generated based on two recombinant inbred line (RI) populations. By analyzing the segregation of 346 AFLPs, 113 IMAs and phenotypic characters on a RI population of 163 individuals derived from the cross Védrantais x PI 161375, a first map was constructed. About 20% of the molecular markers were skewed, and the residual heterozygosity was estimated at 4.43% which was not significantly different from the theoretical value of 4.2%. The genome distribution of molecular markers among the 12 linkage groups was not different from a random distribution with the exception of linkage group XII which was found significantly less populated. The genome distributions of IMAs and AFLPs were complementary. AFLPs were found mainly in the middle of each linkage group and sometimes clustered, whereas IMAs were found mainly at the end. A total of 318 molecular markers, mainly AFLP and IMA markers, were mapped on 63 RIs of the second population, Védrantais x PI 414723. Comparison of the maps enables one to conclude that AFLPs and IMAs of like molecular size, amplified with the same primer combination, correspond to the same genetic locus. Both maps were joined through 116 common markers comprising 106 comigrating AFLPs/IMAs, plus five SSRs and five phenotypic markers. The integrated melon map contained 668 loci issuing from the segregation of 1,093 molecular markers in the two RI populations. The composite map spanned 1,654 cM on 12 linkage groups which is the haploid number of chromosomes in melon. Thirty two known-function probes, i.e. known-function genes (9) and morphological traits (23), were included in this map. In addition, the composite map was anchored to previously published maps through SSRs, RFLPs and phenotypic characters.
Scientific Publication
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