Co-Authors:
Amutha, S., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India
Ganapathi, A., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India
Muruganantham, M., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India
Abstract:
In vitro organogenesis was achieved from calluses derived from cotyledon and hypocotyl explants of Vigna radiata on MS medium. Organogenic calluses were induced from both cotyledon and hypocotyl explants excised from 3-day-old seedlings on MS medium containing NAA (1.07 μM and BA (2.22 μM) and 2,4-D (0.90 μM) and BA (2.22 μM) combinations respectively. Regeneration of adventitious shoots from cotyledon derived callus was achieved when they were cultured on MS medium supplemented with NAA (1.07 μM), BA (8.88 μM) and 10% coconut water. Hypocotyl derived calluses produced adventitious shoots when cultured on MS medium fortified with BA (6.66 μM), TDZ (2.5 μM) and 10% coconut water. Addition of GA3 at 1.73 μM favored maximum elongation of shoots. Regenerated shoots produced prominent roots when transferred to half strength MS medium supplemented with 4.90 μM IBA. Rooted plantlets, thus developed were hardened and successfully established in field. Among the different carbohydrates and media tested, 87.64 mM sucrose and MS+B5 medium proved best for maximum production of shoots. This protocol produced an average of seven plants per hypocotyl derived callus and 15 plants per cotyledon derived callus over a period of 3 months.
תפריט נגישות
ניגודיות עדינה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
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