Co-Authors:
Yalcin-Mendi, N.Y., Department of Horticulture, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey
Ipek, M.
Kacan, H., Department of Horticulture, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey
Curuk, S., Department of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, 31034, Antakya, Turkey
Sari, N., Department of Horticulture, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey
Cetiner, S., Fac. of Eng. and Natural Sciences, University of Sabanci, 81474 Tuzla, Istanbul, Turkey
Gaba, V., Department of Virology, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel
Abstract:
The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of watermelon [Citrullus lanatus (Thunb) Matsum & Nakai] cv Crimson Sweet were cultured on MS medium containing combinations of benzyl adenine (BA) (0, 5, 10, 20 μM) and indole-3-acetic acid (IAA) (0, 0.5, 5 μM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained from the media containing 10 μM BA + 0.5 μM IAA and 20 μM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12days in culture which later developed into buds.
תפריט נגישות
ניגודיות עדינה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
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