נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The presence of Drosophila melanogaster sex peptide-like immunoreactivity in the accessory glands of male Helicoverpa armigera
Year:
2004
Source of publication :
Journal of Insect Physiology
Authors :
נגלקשמי, וידיה
;
.
רפאלי, עדה
;
.
Volume :
50
Co-Authors:
Nagalakshmi, V.K., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Applebaum, S.W., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel
Kubli, E., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Choffat, Y., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Rafaeli, A., Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
241
To page:
248
(
Total pages:
8
)
Abstract:
In this study a highly specific polyclonal antibody to DrmSP was produced and used to develop and standardize a sensitive direct ELISA. Structure-activity studies revealed that the antiserum is specific to the N-terminal of DrmSP. This ELISA was used for the detection of DrmSP-like immunoreactivity in the reproductive tissues of male Helicoverpa armigera moths at femtomole levels. Two positive immunoreactive peaks were found in HPLC purified extracts of male accessory glands. The immunoreactive peak, which contained a higher amount of immunoreactivity, was also found to be pheromonostatic in PBAN-injected decapitated females as well as in intact female moths during their peak pheromone production. Lower levels of DrmSP-like immunoreactivity were found in younger males (1-2 day-old) when compared to older males (3-7 day-old). © 2003 Elsevier Ltd. All rights reserved.
Note:
Related Files :
Animals
Female
Lepidoptera
Male
metabolism
peptides
pheromones
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.jinsphys.2003.12.002
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19747
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
You may also be interested in
Scientific Publication
The presence of Drosophila melanogaster sex peptide-like immunoreactivity in the accessory glands of male Helicoverpa armigera
50
Nagalakshmi, V.K., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Applebaum, S.W., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel
Kubli, E., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Choffat, Y., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Rafaeli, A., Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
The presence of Drosophila melanogaster sex peptide-like immunoreactivity in the accessory glands of male Helicoverpa armigera
In this study a highly specific polyclonal antibody to DrmSP was produced and used to develop and standardize a sensitive direct ELISA. Structure-activity studies revealed that the antiserum is specific to the N-terminal of DrmSP. This ELISA was used for the detection of DrmSP-like immunoreactivity in the reproductive tissues of male Helicoverpa armigera moths at femtomole levels. Two positive immunoreactive peaks were found in HPLC purified extracts of male accessory glands. The immunoreactive peak, which contained a higher amount of immunoreactivity, was also found to be pheromonostatic in PBAN-injected decapitated females as well as in intact female moths during their peak pheromone production. Lower levels of DrmSP-like immunoreactivity were found in younger males (1-2 day-old) when compared to older males (3-7 day-old). © 2003 Elsevier Ltd. All rights reserved.
Scientific Publication
You may also be interested in