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חיפוש מתקדם
Journal of Insect Physiology
Nagalakshmi, V.K., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Applebaum, S.W., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel
Kubli, E., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Choffat, Y., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Rafaeli, A., Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
In this study a highly specific polyclonal antibody to DrmSP was produced and used to develop and standardize a sensitive direct ELISA. Structure-activity studies revealed that the antiserum is specific to the N-terminal of DrmSP. This ELISA was used for the detection of DrmSP-like immunoreactivity in the reproductive tissues of male Helicoverpa armigera moths at femtomole levels. Two positive immunoreactive peaks were found in HPLC purified extracts of male accessory glands. The immunoreactive peak, which contained a higher amount of immunoreactivity, was also found to be pheromonostatic in PBAN-injected decapitated females as well as in intact female moths during their peak pheromone production. Lower levels of DrmSP-like immunoreactivity were found in younger males (1-2 day-old) when compared to older males (3-7 day-old). © 2003 Elsevier Ltd. All rights reserved.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
The presence of Drosophila melanogaster sex peptide-like immunoreactivity in the accessory glands of male Helicoverpa armigera
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Nagalakshmi, V.K., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Applebaum, S.W., Department of Entomology, Hebrew University, P.O. Box 12, Rehovot 76100, Israel
Kubli, E., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Choffat, Y., Zoological Institute, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
Rafaeli, A., Inst. Technol. Storage Agric. Prod., ARO, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
The presence of Drosophila melanogaster sex peptide-like immunoreactivity in the accessory glands of male Helicoverpa armigera
In this study a highly specific polyclonal antibody to DrmSP was produced and used to develop and standardize a sensitive direct ELISA. Structure-activity studies revealed that the antiserum is specific to the N-terminal of DrmSP. This ELISA was used for the detection of DrmSP-like immunoreactivity in the reproductive tissues of male Helicoverpa armigera moths at femtomole levels. Two positive immunoreactive peaks were found in HPLC purified extracts of male accessory glands. The immunoreactive peak, which contained a higher amount of immunoreactivity, was also found to be pheromonostatic in PBAN-injected decapitated females as well as in intact female moths during their peak pheromone production. Lower levels of DrmSP-like immunoreactivity were found in younger males (1-2 day-old) when compared to older males (3-7 day-old). © 2003 Elsevier Ltd. All rights reserved.
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