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פותח על ידי קלירמאש פתרונות בע"מ -
Molecular comparisons of entomopathogenic nematodes using randomly amplified polymorphic DNA (RAPD) markers [Comparaison moleculaire entre nematodes entomopathogenes grace a des marqueurs polymorphes d'ADN amplifies au hasard (RAPD)]
Year:
1996
Source of publication :
Fundamental and Applied Nematology
Authors :
גלזר, איתמר
;
.
Volume :
19
Co-Authors:
Hashmi, G., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Glazer, I., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Gaugler, R., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Facilitators :
From page:
399
To page:
406
(
Total pages:
8
)
Abstract:
The two entomopathogenic nematode genera Heterorhabditis and Steinernema were examined for genetic relatedness by random amplified polymorphic DNA (RAPD) markers. Species of these genera have great potential for biological control of insect pests, and rapid and accurate identification of the many isolates of these nematodes is important for their implementation. Eighty ten-mer random primers were screened for use in determining genetic variability among isolates of Heterorhabditis bacteriophora, H. megidis, Steinernema glaseri, S. carpocapsae, and S. feltiae. A similarity of 0-6% was observed between the members of different genera, and less than 20% similarity was observed among congeneric species. Genetic analysis using seven primers of different Heterorhabditis isolates from various parts of the world showed 24-96% similarity. These results agreed with other reports which showed many genetic differences among isolates. RAPD analysis described here can be used for the assessment of genetic variability among species and isolates of entomopathogenic nematodes.
Note:
Related Files :
Entomopathogenic nematodes
Insecta
Nematoda
RAPD
Steinernema carpocapsae
Steinernema feltiae
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:

Itamar's own PDF.

ID:
19832
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:31
Scientific Publication
Molecular comparisons of entomopathogenic nematodes using randomly amplified polymorphic DNA (RAPD) markers [Comparaison moleculaire entre nematodes entomopathogenes grace a des marqueurs polymorphes d'ADN amplifies au hasard (RAPD)]
19
Hashmi, G., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Glazer, I., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Gaugler, R., Department of Entomology, Cook College, New Jersey Experimental Station, New Brunswick, NJ 08903, United States
Molecular comparisons of entomopathogenic nematodes using randomly amplified polymorphic DNA (RAPD) markers [Comparaison moleculaire entre nematodes entomopathogenes grace a des marqueurs polymorphes d'ADN amplifies au hasard (RAPD)]
The two entomopathogenic nematode genera Heterorhabditis and Steinernema were examined for genetic relatedness by random amplified polymorphic DNA (RAPD) markers. Species of these genera have great potential for biological control of insect pests, and rapid and accurate identification of the many isolates of these nematodes is important for their implementation. Eighty ten-mer random primers were screened for use in determining genetic variability among isolates of Heterorhabditis bacteriophora, H. megidis, Steinernema glaseri, S. carpocapsae, and S. feltiae. A similarity of 0-6% was observed between the members of different genera, and less than 20% similarity was observed among congeneric species. Genetic analysis using seven primers of different Heterorhabditis isolates from various parts of the world showed 24-96% similarity. These results agreed with other reports which showed many genetic differences among isolates. RAPD analysis described here can be used for the assessment of genetic variability among species and isolates of entomopathogenic nematodes.
Scientific Publication
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