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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The Clavibacter michiganensis subsp. michiganensis -tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection
Year:
2012
Source of publication :
Journal of Proteome Research
Authors :
מנוליס-ששון, שולמית
;
.
צ'לופוביץ, לאורה
;
.
Volume :
11
Co-Authors:
Savidor, A., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Gartemann, K.-H., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Eichenlaub, R., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Tews, H., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Mayer, K., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Giannone, R.J., Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States
Hettich, R.L., Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States
Sessa, G., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
736
To page:
750
(
Total pages:
15
)
Abstract:
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) causes wilt and canker disease of tomato (Solanum lycopersicum). Mechanisms of Cmm pathogenicity and tomato response to Cmm infection are not well understood. To explore the interaction between Cmm and tomato, multidimensional protein identification technology (MudPIT) and tandem mass spectrometry were used to analyze in vitro and in planta generated samples. The results show that during infection Cmm senses the plant environment, transmits signals, induces, and then secretes multiple hydrolytic enzymes, including serine proteases of the Pat-1, Ppa, and Sbt familes, the CelA, XysA, and NagA glycosyl hydrolases, and other cell wall-degrading enzymes. Tomato induction of pathogenesis-related (PR) proteins, LOX1, and other defense-related proteins during infection indicates that the plant senses the invading bacterium and mounts a basal defense response, although partial with some suppressed components including class III peroxidases and a secreted serine peptidase. The tomato ethylene-synthesizing enzyme ACC-oxidase was induced during infection with the wild-type Cmm but not during infection with an endophytic Cmm strain, identifying Cmm-triggered host synthesis of ethylene as an important factor in disease symptom development. The proteomic data were also used to improve Cmm genome annotation, and thousands of Cmm gene models were confirmed or expanded. © 2011 American Chemical Society.
Note:
Related Files :
Actinomycetales
chromosomal homolog of pat 1E
enzyme release
ethylene
Plant Diseases
proteomics
unclassified drug
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/pr200646a
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
19852
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:32
You may also be interested in
Scientific Publication
The Clavibacter michiganensis subsp. michiganensis -tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection
11
Savidor, A., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Gartemann, K.-H., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Eichenlaub, R., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Tews, H., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Mayer, K., Department of Genetechnology/Microbiology, Faculty of Biology, University of Bielefeld, 33501 Bielefeld, Germany
Giannone, R.J., Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States
Hettich, R.L., Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States
Sessa, G., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
The Clavibacter michiganensis subsp. michiganensis -tomato interactome reveals the perception of pathogen by the host and suggests mechanisms of infection
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) causes wilt and canker disease of tomato (Solanum lycopersicum). Mechanisms of Cmm pathogenicity and tomato response to Cmm infection are not well understood. To explore the interaction between Cmm and tomato, multidimensional protein identification technology (MudPIT) and tandem mass spectrometry were used to analyze in vitro and in planta generated samples. The results show that during infection Cmm senses the plant environment, transmits signals, induces, and then secretes multiple hydrolytic enzymes, including serine proteases of the Pat-1, Ppa, and Sbt familes, the CelA, XysA, and NagA glycosyl hydrolases, and other cell wall-degrading enzymes. Tomato induction of pathogenesis-related (PR) proteins, LOX1, and other defense-related proteins during infection indicates that the plant senses the invading bacterium and mounts a basal defense response, although partial with some suppressed components including class III peroxidases and a secreted serine peptidase. The tomato ethylene-synthesizing enzyme ACC-oxidase was induced during infection with the wild-type Cmm but not during infection with an endophytic Cmm strain, identifying Cmm-triggered host synthesis of ethylene as an important factor in disease symptom development. The proteomic data were also used to improve Cmm genome annotation, and thousands of Cmm gene models were confirmed or expanded. © 2011 American Chemical Society.
Scientific Publication
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