Genetics Selection Evolution
David, L., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel, Department of Biological Sciences, Stanford University, Stanford, CA 94305, United States
Rosenberg, N.A., Department of Human Genetics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2218, United States
Lavi, U., Institute of Horticulture, ARO-Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Feldman, M.W., Department of Biological Sciences, Stanford University, Stanford, CA 94305, United States
Hillel, J., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Genetic relationships among eight populations of domesticated carp (Cyprinus carpio L.), a species with a partially duplicated genome, were studied using 12 microsatellites and 505 AFLP bands. The populations included three aquacultured carp strains and five ornamental carp (koi) variants. Grass carp (Ctenopharyngodon idella) was used as an outgroup. AFLP-based gene diversity varied from 5% (grass carp) to 32% (koi) and reflected the reasonably well understood histories and breeding practices of the populations. A large fraction of the molecular variance was due to differences between aquacultured and ornamental carps. Further analyses based on microsatellite data, including cluster analysis and neighbor-joining trees, supported the genetic distinctiveness of aquacultured and ornamental carps, despite the recent divergence of the two groups. In contrast to what was observed for AFLP-based diversity, the frequency of heterozygotes based on microsatellites was comparable among all populations. This discrepancy can potentially be explained by duplication of some loci in Cyprinus carpio L., and a model that shows how duplication can increase heterozygosity estimates for microsatellites but not for AFLP loci is discussed. Our analyses in carp can help in understanding the consequences of genotyping duplicated loci and in interpreting discrepancies between dominant and co-dominant markers in species with recent genome duplication. © INRA, EDP Sciences, 2007.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Genetic diversity and population structure inferred from the partially duplicated genome of domesticated carp, Cyprinus carpio L.
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David, L., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel, Department of Biological Sciences, Stanford University, Stanford, CA 94305, United States
Rosenberg, N.A., Department of Human Genetics, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2218, United States
Lavi, U., Institute of Horticulture, ARO-Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Feldman, M.W., Department of Biological Sciences, Stanford University, Stanford, CA 94305, United States
Hillel, J., Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Genetic diversity and population structure inferred from the partially duplicated genome of domesticated carp, Cyprinus carpio L.
Genetic relationships among eight populations of domesticated carp (Cyprinus carpio L.), a species with a partially duplicated genome, were studied using 12 microsatellites and 505 AFLP bands. The populations included three aquacultured carp strains and five ornamental carp (koi) variants. Grass carp (Ctenopharyngodon idella) was used as an outgroup. AFLP-based gene diversity varied from 5% (grass carp) to 32% (koi) and reflected the reasonably well understood histories and breeding practices of the populations. A large fraction of the molecular variance was due to differences between aquacultured and ornamental carps. Further analyses based on microsatellite data, including cluster analysis and neighbor-joining trees, supported the genetic distinctiveness of aquacultured and ornamental carps, despite the recent divergence of the two groups. In contrast to what was observed for AFLP-based diversity, the frequency of heterozygotes based on microsatellites was comparable among all populations. This discrepancy can potentially be explained by duplication of some loci in Cyprinus carpio L., and a model that shows how duplication can increase heterozygosity estimates for microsatellites but not for AFLP loci is discussed. Our analyses in carp can help in understanding the consequences of genotyping duplicated loci and in interpreting discrepancies between dominant and co-dominant markers in species with recent genome duplication. © INRA, EDP Sciences, 2007.
Scientific Publication