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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Cloning and characterization of micro-RNAs from moss
Year:
2005
Source of publication :
Plant Journal
Authors :
ארזי, צחי
;
.
טלמור-ניימן, מלי
;
.
סתו, רן
;
.
Volume :
43
Co-Authors:
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Talmor-Neiman, M., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Stav, R., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Riese, M., Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany
Huijser, P., Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany
Baulcombe, D.C., Sainsbury Laboratory, Colney Lane, Norwich NR4 7UH, United Kingdom
Facilitators :
From page:
837
To page:
848
(
Total pages:
12
)
Abstract:
Micro-RNAs (miRNAs) are one class of endogenous tiny RNAs that play important regulatory roles in plant development and responses to external stimuli. To date, miRNAs have been cloned from higher plants such as Arabidopsis, rice and pumpkin, and there is limited information on their identity in lower plants including Bryophytes. Bryophytes are among the oldest groups of land plants among the earth's flora, and are important for our understanding of the transition to life on land. To identify miRNAs that might have played a role early in land plant evolution, we constructed a library of small RNAs from the juvenile gametophyte (protonema) of the moss Physcomitrella patens. Sequence analysis revealed five higher plant miRNA homologuesq2, including three members of the miR319 family, previously shown to be involved in the regulation of leaf morphogenesis, and miR156, which has been suggested to regulate several members of the SQUAMOSA PROMOTER BINDING-LIKE (SPL) family in Arabidopsis. We have cloned PpSBP3, a moss SPL homologue that contains an miR156 complementary site, and demonstrated that its mRNA is cleaved within that site suggesting that it is an miR156 target in moss. Six additional candidate moss miRNAs were identified and shown to be expressed in the gametophyte, some of which were developmentally regulated or upregulated by auxin. Our observations suggest that miRNAs play important regulatory roles in mosses. © 2005 Blackwell Publishing Ltd.
Note:
Related Files :
arabidopsis
Genes
Genetics
metabolism
moss
Nucleic acids
Plants
RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2005.02499.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20017
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:33
Scientific Publication
Cloning and characterization of micro-RNAs from moss
43
Arazi, T., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Talmor-Neiman, M., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Stav, R., Department of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Riese, M., Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany
Huijser, P., Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany
Baulcombe, D.C., Sainsbury Laboratory, Colney Lane, Norwich NR4 7UH, United Kingdom
Cloning and characterization of micro-RNAs from moss
Micro-RNAs (miRNAs) are one class of endogenous tiny RNAs that play important regulatory roles in plant development and responses to external stimuli. To date, miRNAs have been cloned from higher plants such as Arabidopsis, rice and pumpkin, and there is limited information on their identity in lower plants including Bryophytes. Bryophytes are among the oldest groups of land plants among the earth's flora, and are important for our understanding of the transition to life on land. To identify miRNAs that might have played a role early in land plant evolution, we constructed a library of small RNAs from the juvenile gametophyte (protonema) of the moss Physcomitrella patens. Sequence analysis revealed five higher plant miRNA homologuesq2, including three members of the miR319 family, previously shown to be involved in the regulation of leaf morphogenesis, and miR156, which has been suggested to regulate several members of the SQUAMOSA PROMOTER BINDING-LIKE (SPL) family in Arabidopsis. We have cloned PpSBP3, a moss SPL homologue that contains an miR156 complementary site, and demonstrated that its mRNA is cleaved within that site suggesting that it is an miR156 target in moss. Six additional candidate moss miRNAs were identified and shown to be expressed in the gametophyte, some of which were developmentally regulated or upregulated by auxin. Our observations suggest that miRNAs play important regulatory roles in mosses. © 2005 Blackwell Publishing Ltd.
Scientific Publication
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